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The Role of Metabotropic Glutamate Receptor 5 on the Stromal Cell-Derived Factor-1/CXCR4 System in Oral Cancer
https://tokushima-u.repo.nii.ac.jp/records/2000195
https://tokushima-u.repo.nii.ac.jp/records/2000195ce26a374-3625-425c-94d0-65f6695f83f4
| 名前 / ファイル | ライセンス | アクション |
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pone_8_11_e80773.pdf (1.9 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||
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| 公開日 | 2024-10-09 | |||||||||||||||||||
| アクセス権 | ||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||
| item_1722929371688 | ||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||
| 関連識別子 | https://doi.org/10.1371/journal.pone.0080773 | |||||||||||||||||||
| 言語 | ja | |||||||||||||||||||
| 関連名称 | 10.1371/journal.pone.0080773 | |||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||
| タイトル | ||||||||||||||||||||
| タイトル | The Role of Metabotropic Glutamate Receptor 5 on the Stromal Cell-Derived Factor-1/CXCR4 System in Oral Cancer | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| タイトル別表記 | ||||||||||||||||||||
| その他のタイトル | Role of mGluR5 in Oral Cancer | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 著者 |
栗林, 伸行
× 栗林, 伸行× 内田, 大亮× Kinouchi, Makoto
× 髙丸, 菜都美
WEKO
411
× 玉谷, 哲也× 永井, 宏和× 宮本, 洋二
WEKO
1703
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| 抄録 | ||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||
| 内容記述 | We have demonstrated that blocking CXCR4 may be a potent anti-metastatic therapy for CXCR4-related oral cancer. However, as CXCR4 antagonists are currently in clinical use to induce the mobilization of hematopoietic stem cells, continuous administration as an inhibitor for the metastasis may lead to persistent leukocytosis. In this study, we investigated the novel therapeutic downstream target(s) of the SDF-1/CXCR4 system, using B88-SDF-1 cells, which have an autocrine SDF-1/CXCR4 system and exhibit distant metastatic potential in vivo. Microarray analysis revealed that 418 genes were upregulated in B88-SDF-1 cells. We identified a gene that is highly upregulated in B88-SDF-1 cells, metabotropic glutamate receptor 5 (mGluR5), which was downregulated following treatment with 1,1’ -[1,4-Phenylenebis(methylene)]bis-1,4,8,11-tetraazacyclotetradecane octahydrochloride (AMD3100), a CXCR4 antagonist. The upregulation of mGluR5 mRNA in the SDF-1/CXCR4 system was predominately regulated by the Ras-extracellular signal-regulated kinase (ERK)1/2 pathway. Additionally, the growth of B88-SDF-1 cells was not affected by the mGluR5 agonist (S)-3,5-DHPG (DHPG) or the mGluR5 antagonists 2-Methyl-6-(phenylethynyl)pyridine (MPEP) and 3-((2-Methyl-1,3-thiazol-4-yl)ethynyl)pyridine (MTEP). However, we observed that DHPG promoted B88-SDF-1 cell migration, whereas both MPEP and MTEP inhibited B88-SDF-1 cell migration. To assess drug toxicity, the antagonists were intraperitoneally injected into immunocompetent mice for 4 weeks. Mice injected with MPEP (5 mg/kg) and MTEP (5 mg/kg) did not exhibit any side effects, such as hematotoxicity, allergic reactions or weight loss. The administration of antagonists significantly inhibited the metastasis of B88-SDF-1 cells to the lungs of nude mice. These results suggest that blocking mGluR5 with antagonists such as MPEP and MTEP could prevent metastasis in CXCR4-related oral cancer without causing side effects. | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| bibliographic_information |
en : PLOS ONE 巻 8, 号 11, p. e80773, 発行日 2013-11-13 |
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| 収録物識別子タイプ | EISSN | |||||||||||||||||||
| 収録物識別子 | 19326203 | |||||||||||||||||||
| 出版者 | ||||||||||||||||||||
| 出版者 | PLOS | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| item_10001_rights_15 | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 権利情報 | This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||||||||||||||||
| item_1723180141928 | ||||||||||||||||||||
| 識別子 | 273787 | |||||||||||||||||||
| 識別子タイプ | URI | |||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||
