| Item type |
文献 / Documents(1) |
| 公開日 |
2024-10-15 |
| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1371/journal.pone.0027222 |
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関連名称 |
10.1371/journal.pone.0027222 |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Glycolysis Inhibition Inactivates ABC Transporters to Restore Drug Sensitivity in Malignant Cells |
| タイトル別表記 |
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その他のタイトル |
Glycolysis Inhibition Overcomes Drug Resistance |
| 著者 |
Nakano, Ayako
辻, 大輔
三木, 浩和
Cui, Qu
El Sayed, Salah Mohamed
Ikegame, Akishige
Oda, Asuka
Amou, Hiroe
中村, 信元
原田, 武志
藤井, 志朗
Kagawa, Kumiko
Takeuchi, Kyoko
Sakai, Akira
Ozaki, Shuji
Okano, Kazuma
Nakamura, Takahiro
Itoh, Kohji
松本, 俊夫
安倍, 正博
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| 抄録 |
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内容記述 |
Cancer cells eventually acquire drug resistance largely via the aberrant expression of ATP-binding cassette (ABC) transporters, ATP-dependent efflux pumps. Because cancer cells produce ATP mostly through glycolysis, in the present study we explored the effects of inhibiting glycolysis on the ABC transporter function and drug sensitivity of malignant cells. Inhibition of glycolysis by 3-bromopyruvate (3BrPA) suppressed ATP production in malignant cells, and restored the retention of daunorubicin or mitoxantrone in ABC transporter-expressing, RPMI8226 (ABCG2), KG-1 (ABCB1) and HepG2 cells (ABCB1 and ABCG2). Interestingly, although side population (SP) cells isolated from RPMI8226 cells exhibited higher levels of glycolysis with an increased expression of genes involved in the glycolytic pathway, 3BrPA abolished Hoechst 33342 exclusion in SP cells. 3BrPA also disrupted clonogenic capacity in malignant cell lines including RPMI8226, KG-1, and HepG2. Furthermore, 3BrPA restored cytotoxic effects of daunorubicin and doxorubicin on KG-1 and RPMI8226 cells, and markedly suppressed subcutaneous tumor growth in combination with doxorubicin in RPMI8226-implanted mice. These results collectively suggest that the inhibition of glycolysis is able to overcome drug resistance in ABC transporter-expressing malignant cells through the inactivation of ABC transporters and impairment of SP cells with enhanced glycolysis as well as clonogenic cells. |
| 書誌情報 |
en : PLOS ONE
巻 6,
号 11,
p. e27222,
発行日 2011-11-02
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| 収録物ID |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
19326203 |
| 出版者 |
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出版者 |
PLOS |
| 権利情報 |
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|
権利情報 |
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| EID |
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識別子 |
252725 |
| 言語 |
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言語 |
eng |
pone_6_11_e27222.pdf (1.1 MB)
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