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Comprehensive Enzymatic Analysis of the Cellulolytic System in Digestive Fluid of the Sea Hare Aplysia kurodai. Efficient Glucose Release from Sea Lettuce by Synergistic Action of 45 kDa Endoglucanase and 210 kDa ß-Glucosidase
https://tokushima-u.repo.nii.ac.jp/records/2000258
https://tokushima-u.repo.nii.ac.jp/records/20002581d45deb1-cc8e-4b97-9c25-2e9385369295
| 名前 / ファイル | ライセンス | アクション |
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pone_8_6_e65418.pdf (3.5 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||
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| 公開日 | 2024-10-04 | |||||||||||||||
| アクセス権 | ||||||||||||||||
| アクセス権 | open access | |||||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
| 資源タイプ | journal article | |||||||||||||||
| 出版社版DOI | ||||||||||||||||
| 関連識別子 | https://doi.org/10.1371/journal.pone.0065418 | |||||||||||||||
| 関連名称 | 10.1371/journal.pone.0065418 | |||||||||||||||
| 出版タイプ | ||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | Comprehensive Enzymatic Analysis of the Cellulolytic System in Digestive Fluid of the Sea Hare Aplysia kurodai. Efficient Glucose Release from Sea Lettuce by Synergistic Action of 45 kDa Endoglucanase and 210 kDa ß-Glucosidase | |||||||||||||||
| タイトル別表記 | ||||||||||||||||
| その他のタイトル | Sea Hare Cellulose Digestion System | |||||||||||||||
| 著者 |
辻, 明彦
× 辻, 明彦
WEKO
121
× Tominaga, Keiko
× Nishiyama, Nami
× 湯浅, 恵造 |
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| 抄録 | ||||||||||||||||
| 内容記述 | Although many endo-ß-1,4-glucanases have been isolated in invertebrates, their cellulolytic systems are not fully understood. In particular, gastropod feeding on seaweed is considered an excellent model system for production of bioethanol and renewable bioenergy from third-generation feedstocks (microalgae and seaweeds). In this study, enzymes involved in the conversion of cellulose and other polysaccharides to glucose in digestive fluids of the sea hare (Aplysia kurodai) were screened and characterized to determine how the sea hare obtains glucose from sea lettuce (Ulva pertusa). Four endo-ß-1,4-glucanases (21 K, 45 K, 65 K, and 95 K cellulase) and 2 ß-glucosidases (110 K and 210 K) were purified to a homogeneous state, and the synergistic action of these enzymes during cellulose digestion was analyzed. All cellulases exhibited cellulase and lichenase activities and showed distinct cleavage specificities against cellooligosaccharides and filter paper. Filter paper was digested to cellobiose, cellotriose, and cellotetraose by 21 K cellulase, whereas 45 K and 65 K enzymes hydrolyzed the filter paper to cellobiose and glucose. 210 K ß-glucosidase showed unique substrate specificity against synthetic and natural substrates, and 4-methylumbelliferyl (4MU)-ß-glucoside, 4MU–ß-galactoside, cello-oligosaccharides, laminarin, and lichenan were suitable substrates. Furthermore, 210 K ß-glucosidase possesses lactase activity. Although ß-glucosidase and cellulase are necessary for efficient hydrolysis of carboxymethylcellulose to glucose, laminarin is hydrolyzed to glucose only by 210 K ß-glucosidase. Kinetic analysis of the inhibition of 210 K ß-glucosidase by D-glucono-1,5-lactone suggested the presence of 2 active sites similar to those of mammalian lactase-phlorizin hydrolase. Saccharification of sea lettuce was considerably stimulated by the synergistic action of 45 K cellulase and 210 K ß-glucosidase. Our results indicate that 45 K cellulase and 210 K ß-glucosidase are the core components of the sea hare digestive system for efficient production of glucose from sea lettuce. These findings contribute important new insights into the development of biofuel processing biotechnologies from seaweed. | |||||||||||||||
| 書誌情報 |
en : PLOS ONE 巻 8, 号 6, p. e65418, 発行日 2013-06-06 |
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| 収録物ID | ||||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||||
| 収録物識別子 | 19326203 | |||||||||||||||
| 出版者 | ||||||||||||||||
| 出版者 | PLOS | |||||||||||||||
| 権利情報 | ||||||||||||||||
| 権利情報 | This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||||||||||||
| EID | ||||||||||||||||
| 識別子 | 274172 | |||||||||||||||
| 言語 | ||||||||||||||||
| 言語 | eng | |||||||||||||||
