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Enhancement of osteoclastogenic activity in osteolytic prostate cancer cells by physical contact with osteoblasts
https://tokushima-u.repo.nii.ac.jp/records/2000289
https://tokushima-u.repo.nii.ac.jp/records/2000289a738ad98-11ad-479b-ad11-696f044ac3e4
| 名前 / ファイル | ライセンス | アクション |
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bjc_104_3_505.pdf (740 KB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||||||||||||||
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| 公開日 | 2024-10-18 | |||||||||||||||||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||||||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1038/sj.bjc.6606070 | |||||||||||||||||||||||||||||||||||
| 関連名称 | 10.1038/sj.bjc.6606070 | |||||||||||||||||||||||||||||||||||
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| 出版タイプ | VoR | |||||||||||||||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||||||||||||||
| タイトル | Enhancement of osteoclastogenic activity in osteolytic prostate cancer cells by physical contact with osteoblasts | |||||||||||||||||||||||||||||||||||
| タイトル別表記 | ||||||||||||||||||||||||||||||||||||
| その他のタイトル | Osteoclastogenesis in prostate cancer by cell-cell contact | |||||||||||||||||||||||||||||||||||
| 著者 |
Shiirevnyamba, A
× Shiirevnyamba, A
× 高橋, 徹行× Shan, H
× 小川, 博久
WEKO
310
× Yano, S
× 金山, 博臣
WEKO
1546
× 泉, 啓介
WEKO
1530
× 上原, 久典
WEKO
1548
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| 抄録 | ||||||||||||||||||||||||||||||||||||
| 内容記述 | BACKGROUND: The interaction between prostate cancer cells and osteoblasts is critical for the development of bone metastasis. Metastatic cancer cells may physically contact osteoblasts in the bone microenvironment; however, the biological significance of this interaction is not fully understood. METHODS: Human prostate cancer cells (the osteolytic cell line PC-3 and the osteoblastic cell line MDA-PCa 2b) and human osteoblasts (hFOB1.19) were cocultured under two different conditions (bilayer and contact conditions). Differential gene expression profiles of prostate cancer cells were then investigated using microarray analysis. Differentially expressed genes were analysed using RT–PCR and western blotting, and the effect of anti-cadherin neutralising antibodies on their expression was assayed. The osteoclastogenic activity of cells grown under these different conditions was also investigated using an in vitro assay. RESULTS: When PC-3 or MDA-PCa 2b cells were cocultured with hFOB1.19 cells under contact conditions, the expression of eight genes was upregulated and that of one gene was downregulated in PC-3 cells compared with gene expression in bilayer culture. No differentially expressed genes were detected in MDA-PCa 2b cells. Four of the eight upregulated genes (interleukin-1β (IL-1β), cyclooxygenase-2 (COX-2), IL-6 and the third component of complement (C3)) have already been reported to participate in osteoclastogenesis. Indeed, a cell lysate of PC-3 cells grown under contact coculture conditions significantly enhanced osteoclastogenesis in vitro (P<0.005). neutralisation of cadherin-11 with a specific antibody inhibited upregulation of COX-2 and C3 mRNA in PC-3 cells. In contrast, neutralisation of N-cadherin induced upregulation of COX-2 mRNA. CONCLUSION: Physical contact between osteolytic prostate cancer cells and osteoblasts may upregulate osteoclastogenesis-related gene expression in prostate cancer cells and enhance osteoclastogenesis. Additionally, cadherin-11 and N-cadherin are involved in this process. These data provide evidence supporting new therapies of prostate cancer bone metastasis that target direct cancer-cell-osteoblast cell–cell contact. |
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| キーワード | ||||||||||||||||||||||||||||||||||||
| 主題 | physical contact | |||||||||||||||||||||||||||||||||||
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| 主題 | cell-to-cell interaction | |||||||||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||||||||
| 主題 | prostate cancer | |||||||||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||||||||
| 主題 | bone metastasis | |||||||||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||||||||
| 主題 | osteoclastogenesis | |||||||||||||||||||||||||||||||||||
| 書誌情報 |
en : British Journal of Cancer 巻 104, 号 3, p. 505-513, 発行日 2011-01-04 |
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| 収録物識別子タイプ | EISSN | |||||||||||||||||||||||||||||||||||
| 収録物識別子 | 15321827 | |||||||||||||||||||||||||||||||||||
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| 収録物識別子タイプ | PISSN | |||||||||||||||||||||||||||||||||||
| 収録物識別子 | 00070920 | |||||||||||||||||||||||||||||||||||
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| 出版者 | Springer Nature | |||||||||||||||||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||||||||||||||||
| 権利情報 | From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. | |||||||||||||||||||||||||||||||||||
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| 識別子 | 224164 | |||||||||||||||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||||||||||||||
