| Item type |
文献 / Documents(1) |
| 公開日 |
2024-11-12 |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.1016/j.heliyon.2024.e27456 |
|
|
関連名称 |
10.1016/j.heliyon.2024.e27456 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Development of a standard evaluation method for microbial UV sensitivity using light-emitting diodes |
| 著者 |
石田, 快
Onoda, Yushi
Kadomura-Ishikawa, Yasuko
Nagahashi, Miharu
Yamashita, Michiyo
Fukushima, Shiho
Aizawa, Toshihiko
Yamauchi, Shigeharu
Fujikawa, Yasuo
Tanaka, Tomotake
上番増, 喬
芥川, 正武
馬渡, 一諭
髙橋, 章
|
| 抄録 |
|
|
内容記述 |
Ultraviolet (UV) light is an effective disinfection method. In particular, UV light-emitting diodes (UV-LEDs) are expected to have many applications as light sources owing to their compact form factor and wide range of choices of wavelengths. However, the UV sensitivity of microorganisms for each UV wavelength has not been evaluated comprehensively because standard experimental conditions based on LED characteristics have not been established. Therefore, it is necessary to establish a standard evaluation method based on LED characteristics. Here, we developed a new UV-LED device based on strictly controlled irradiation conditions using LEDs for each wavelength (250–365 nm), checked the validity of the device characteristics and evaluated the UV sensitivity of Escherichia coli using this new evaluation method. For this new device, we considered accurate irradiance, accurate spectra, irradiance uniformity, accurate dose, beam angle, surrounding material reflections, and sample condition. From our results, the following UV irradiation conditions were established as standard: 1 mW/cm2 irradiance, bacterial solution with absorbance value of A600 = 0.5 diluted 10 times solution, solution volume of 1 mL, working distance (WD) of 100 mm. In order to compare the effects of irradiation under uniform conditions on inactivation of microorganisms, we assessed inactivation effect of E. coli by LED irradiation at each wavelength using the U280 LED as a standard wavelength. The inactivation effect for U280 LED irradiation was −0.95 ± 0.21 log at a dose of 4 mJ/cm2. Under this condition of dose, our results showed a high wavelength dependence of the inactivation effect at each UV wavelength peaking at 267 nm. Our study showed that this irradiation system was validated for the standard UV irradiation system and could be contributed to the establishment of food and water hygiene control methods and the development of equipment for the prevention of infectious diseases. |
| キーワード |
|
|
主題 |
UV-LEDs |
| キーワード |
|
|
主題 |
Escherichia coli |
| キーワード |
|
|
主題 |
UV dose |
| キーワード |
|
|
主題 |
Standard evaluation method |
| キーワード |
|
|
主題 |
Microorganism inactivation |
| 書誌情報 |
en : Heliyon
巻 10,
号 6,
p. e27456,
発行日 2024-03-08
|
| 収録物ID |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
24058440 |
| 出版者 |
|
|
出版者 |
Elsevier |
| 権利情報 |
|
|
権利情報 |
This is an open access article under the CC BY license. |
| EID |
|
|
識別子 |
407372 |
| 言語 |
|
|
言語 |
eng |
heliyon_10_6_e27456.pdf (5.1 MB)
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