| Item type |
文献 / Documents(1) |
| 公開日 |
2025-02-20 |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.celrep.2014.05.056 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1016/j.celrep.2014.05.056 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
In Vivo Visualization of Subtle, Transient, and Local Activity of Astrocytes Using an Ultrasensitive Ca2+ Indicator |
|
言語 |
en |
| 著者 |
Kanemaru, Kazunori
Sekiya, Hiroshi
Xu, Ming
Satoh, Kaname
Kitajima, Nami
Yoshida, Keitaro
Okubo, Yohei
Sasaki, Takuya
Moritoh, Satoru
Hasuwa, Hidetoshi
Mimura, Masaru
堀川, 一樹
Matsui, Ko
Nagai, Takeharu
Iino, Masamitsu
Tanaka, Kenji F.
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Astrocytes generate local calcium (Ca2+) signals that are thought to regulate their functions. Visualization of these signals in the intact brain requires an imaging method with high spatiotemporal resolution. Here, we describe such a method using transgenic mice expressing the ultrasensitive ratiometric Ca2+ indicator yellow Cameleon-Nano 50 (YC-Nano50) in astrocytes. In these mice, we detected a unique pattern of Ca2+ signals. These occur spontaneously, predominantly in astrocytic fine processes, but not the cell body. Upon sensory stimulation, astrocytes initially responded with Ca2+ signals at fine processes, which then propagated to the cell body. These observations suggest that astrocytic fine processes function as a high-sensitivity detector of neuronal activities. Thus, the method provides a useful tool for studying the activity of astrocytes in brain physiology and pathology. |
|
言語 |
en |
| 書誌情報 |
en : Cell Reports
巻 8,
号 1,
p. 311-318,
発行日 2014-06-26
|
| 収録物ID |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
22111247 |
| 収録物ID |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
26391856 |
| 出版者 |
|
|
出版者 |
Elsevier |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). |
| EID |
|
|
識別子 |
300708 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
celrep_8_1_311.pdf (2.9 MB)
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