| Item type |
文献 / Documents(1) |
| 公開日 |
2025-03-04 |
| アクセス権 |
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|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1038/srep05705 |
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関連名称 |
10.1038/srep05705 |
| 出版タイプ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems |
| 著者 |
泰江, 章博
Mitsui, Silvia Naomi
Watanabe, Takahito
Sakuma, Tetsushi
親泊, 政一
Yamamoto, Takashi
野地, 澄晴
三戸, 太郎
田中, 栄二
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| 抄録 |
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内容記述 |
Since the establishment of embryonic stem (ES) cell lines, the combined use of gene targeting with homologous recombination has aided in elucidating the functions of various genes. However, the ES cell technique is inefficient and time-consuming. Recently, two new gene-targeting technologies have been developed: the transcription activator-like effector nuclease (TALEN) system and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) system. In addition to aiding researchers in solving conventional problems, these technologies can be used to induce site-specific mutations in various species for which ES cells have not been established. Here, by targeting the Fgf10 gene through RNA microinjection in one-cell mouse embryos with the TALEN and CRISPR/Cas systems, we produced the known limb-defect phenotypes of Fgf10-deficient embryos at the F0 generation. Compared to the TALEN system, the CRISPR/Cas system induced the limb-defect phenotypes with a strikingly higher efficiency. Our results demonstrate that although both gene-targeting technologies are useful, the CRISPR/Cas system more effectively elicits single-step biallelic mutations in mice. |
| 書誌情報 |
en : Scientific Reports
巻 4,
p. 5705,
発行日 2014-07-16
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| 収録物ID |
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収録物識別子タイプ |
EISSN |
|
収録物識別子 |
20452322 |
| 出版者 |
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出版者 |
Springer Nature |
| 権利情報 |
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|
権利情報 |
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| EID |
|
|
識別子 |
280333 |
| 言語 |
|
|
言語 |
eng |
srep_4_5705.pdf (2.6 MB)
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