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顎下腺における炎症性サイトカインIL-βのプロセッシング
https://tokushima-u.repo.nii.ac.jp/records/2000634
https://tokushima-u.repo.nii.ac.jp/records/20006340061a387-0cb0-46e3-83af-a2166a93ddd2
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00004832539.pdf (1.28 MB)
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| Item type | 文献 / Documents(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-03-08 | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| タイトル | ||||||
| タイトル | 顎下腺における炎症性サイトカインIL-βのプロセッシング | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | ガクカセン ニオケル エンショウセイ サイトカイン IL-β ノ プロセッシング | |||||
| 言語 | ja-Kana | |||||
| タイトル別表記 | ||||||
| その他のタイトル | Processing of IL-1β by Tissue Kallikrein mK13 in the Mouse Submandibular Gland | |||||
| 言語 | en | |||||
| 著者 |
姚, 陳娟
× 姚, 陳娟 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We have shown earlier that the expression of interleukin-1β (IL-1β) mRNA is increased in the submandibular gland (SMG) after injection of lipopolysaccharide (LPS). In this present study we suggest that tissue kallikrein mK13 is a candidate of the processing enzyme for the precursor of IL-1β (pro-IL-1β) in the SMG. High levels of 17.5- and 20-kDa IL-1β proteins were detected by Western blotting in the SMG, and saliva from LPS-injected mice. Despite this fact, pro-IL-1β with a molecular size of 35-kDa was not detected in this tissue. The protein for IL-1β-converting enzyme (ICE) was expressed only at a low level in the SMG as compared with its level in various epithelial tissues or LPS-stimulated macrophages. We detected the strong expression of members of kallikrein family (mK1, mK9, mK13, and mK22) in the SMG but not other tissues. By incubation with mK13, but not with mK1, mK9, nor mK22, the 35-kDa pro-IL-1β was cleaved to give two major products with molecular masses of 17.5- and 22- kDa, which production was inhibited by PMSF, a serine protease inhibitor, but not by ICE inhibitors. The pro-IL-β peptide segment, which includes the putative processing site, was hydrolyzed at Leu^<113> and Leu^<114> by incubation with mK13. The immunohistochemistry and an autonomic therapy experiment showed that IL-1β and kallikrein mK13 were co-localized in the secretory granules of granular convoluted tubular cells. Our present results thus suggest that kallikrein mK13 is a plausible candidate for the processing enzyme for pro-IL-1β in the SMG of mice. | |||||
| 言語 | en | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | IL-β | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Kallikrein | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | processing | |||||
| bibliographic_information |
ja : 四国歯学会雑誌 巻 19, 号 2, p. 177-181, 発行日 2007-01 |
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| 収録物ID | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 09146091 | |||||
| 収録物ID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN10050046 | |||||
| 備考 | ||||||
| 言語 | ja | |||||
| 値 | 公開日:2010年1月24日で登録したコンテンツは、国立情報学研究所において電子化したものです。 | |||||
| item_1723180141928 | ||||||
| 識別子 | 153592 | |||||
| 識別子タイプ | URI | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
