| Item type |
文献 / Documents(1) |
| 公開日 |
2011-11-17 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Fluoromicroscopic detection of myc-tagged GLUT4 on the cell surface. Co-localization of the translocated GLUT4 with rearranged actin by insulin treatment in CHO cells and L6 myotubes |
|
言語 |
en |
| 著者 |
アサヒ, ヨシヒコ
ハヤシ, ヒデキ
Lihong, Wang
蛯名, 洋介
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
We earlier developed a novel method to detect translocation of glucose transporter type 4 (GLUT4) directly, quantitatively and simply using c-MYC epitope-tagged GLUT4 (GLUT4myc) (Kanai F, Nishioka Y, Hayashi H, Kamohara S, Todaka M, Ebina Y:J Biol Chem 268:14523-14526, 1993). We further developed the method to visualize GLUT4myc on the cell surfac by fluorescence microscope using a highly sensitive immunochemical detection system in tissue culture cells stably expressing GLUT4myc. The translocation of GLUT4myc was observed on stimulation with insulin in 3T3-L1 adipocytes, CHO cells and L6 myotubes stably expressing GLUT4myc. Platelet-derived growth factor (PDGF), norepinephrine and bradykinin also triggered GLUT4 translocation in CHO-GLUT4myc cells stably expressing each receptor. To observe the distribution of GLUT4 and actin after insulin treatment, double staining for GLUT4myc and actin was performed. Translocated GLUT4myc on the cell surface was co-localized with rearranged actin in CHO cells and L6 myotubes. This result suggests that a correlation exists between GLUT4 translocation and actin rearrangement. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
GLUT4myc translocation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
actin rearrangement |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
fluorescence microscope |
| 書誌情報 |
en : The journal of medical investigation : JMI
巻 46,
号 3-4,
p. 192-199,
発行日 1999
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| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13431420 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11166929 |
| EID |
|
|
識別子 |
66798 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
LID201111172010.pdf (749 KB)
