| Item type |
文献 / Documents(1) |
| 公開日 |
2011-12-02 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
PCR-dot blot hybridization based on the neuraminidase-encoding gene is useful for detection of Bacteroides fragilis |
|
言語 |
en |
| 著者 |
桑原, 知巳
中山, 治之
ミキ, ツヨシ
片岡, 佳子
有持, 秀喜
大西, 克成
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Bacteroides fragilis is a Gram-negative obligate anaerobe frequently isolated from clinical specimens and sometimes causes severe septicemia in compromised hosts. Increasing interest has been shown in the enterotoxigenicity and drug resistance of B. fragilis in the field of medical microbiology. We previously reported rapid detection of this anaerobe by nested PCR targeting a neuraminidase-encoding gene nanH. In the present study, we synthesized a digoxigenin-labeled oligonucleotide probe, NH1,which is specific for nanH of B. fragilis, and we combined the hybridization assay using NH1with the nanH-PCR to detect this anaerobe in a bacteremia model mice. In the specificity test, the oligonucleotide probe, NH1, hybridized only to amplification products from B. fragilis. PCR-dot blot hybridization based on nanH enabled detection of cells of B. fragilis in blood samples even when the number was as low as 2x103colony-forming units/ml. These findings suggest that PCR-dot blot hybridization targeting nanH is a useful procedure for diagnosis of septicemia caused by B. fragilis when viable cells in blood cannot be detected by the traditional culture techniques. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Bacteroides fragilis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
neuraminidase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
oligonucleotide probe |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
septicemia |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
PCR |
| 書誌情報 |
en : The journal of medical investigation : JMI
巻 48,
号 1-2,
p. 60-65,
発行日 2001
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13431420 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11166929 |
| 備考 |
|
|
言語 |
ja |
|
値 |
データ元 : http://medical.med.tokushima-u.ac.jp/jmi/ |
| EID |
|
|
識別子 |
127928 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
LID201112022001.pdf (249 KB)
