| Item type |
文献 / Documents(1) |
| 公開日 |
2011-12-05 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Toll-like receptor 4 regulates gastric pit cell responses to Helicobacter pylori infection |
| 著者 |
カワハラ, ツカサ
桑野, 由紀
(手嶋)近藤, 茂忠
河合, 智子
二川, 健
岸, 恭一
六反, 一仁
|
| 抄録 |
|
|
内容記述 |
Gastric pit cells express mitogen oxidase1 (Mox1) and essential components for the phogocyte NADPH oxidase (p67-, p47-, p40-, and p22-phoxes). Helicobacter pylori (Hp) lipopolysaccharide (LPS) is a potent up-regulator of the Mox1 oxidase. In this study, we examined the expression levels of several key members of the Toll-like receptor (TLR) family in primary cultures of guinea pig gastric pit cells. These cells expressed the TLR4 mRNA. Immunoblot analysis and immunofluorescence histochemistry with an anti-TLR4 antibody showed that gastric pit cells possessed significant amounts of TLR4 protein preferentially on the plasma membrane. In contrast, the cells did not express the TLR2 and TLR9 transcripts and did not contain detectable amounts of TLR2 protein. Neither peptidoglycan from Staphylococcus aureus nor Hp DNA with the CpG motif up-regulated Mox1 oxidase activity. Hp LPS activated nuclear factor-κB in association with the expression of cyclooxygenase II and tumor necrosis factor α transcripts. These findings suggest that TLR4 may play a crucial role in the initiation of inflammatory responses of gastric pit cells against Hp infection. |
| キーワード |
|
|
主題 |
toll-like receptor |
| キーワード |
|
|
主題 |
gastric pit cell |
| キーワード |
|
|
主題 |
Helicobacter pylori |
| キーワード |
|
|
主題 |
mitogen oxidase1 |
| 書誌情報 |
en : The journal of medical investigation : JMI
巻 48,
号 3-4,
p. 190-197,
発行日 2001
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13431420 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11166929 |
| 備考 |
|
|
値 |
データ元 : http://medical.med.tokushima-u.ac.jp/jmi/ |
| EID |
|
|
識別子 |
134282 |
| 言語 |
|
|
言語 |
eng |
LID201112052009.pdf (173 KB)
