| Item type |
文献 / Documents(1) |
| 公開日 |
2011-08-09 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
中等度好熱性細菌Geobacillus stearothermophilus生育温度変異株におけるタンパク質発現解析 |
| タイトル |
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|
タイトル |
チュウトウド コウネツセイ サイキン Geobacillus stearothermophilus セイイク オンド ヘンイカブ ニ オケル タンパクシツ ハツゲン カイセキ |
| タイトル別表記 |
|
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その他のタイトル |
Protein Expression Analysis in Temparature-adaptated Mutant Strains of Moderately thermophile Geobacillus stearothermophilus |
| 著者 |
京極, 仁美
山口, 安亮
小野(宮野), 百代
山口, 綾野
佐藤, 高則
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| 抄録 |
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|
内容記述 |
Optimum growth of moderately thermophile Geobacillus stearothermophilus (Bst) has been observed at 55ºC, and suppressed by the downshift to lower temperature. In order to understand the relationship between growth temperature and protein expression, we made Bst mutant strains which were adapted to lower temperature (30ºC) compared with wild type strain, and then we examined their protein expression under various growth temperatures. When they were cultured at 55ºC, different expression patterns of six proteins (P1-P6) were observed between wild type and mutant strains, whereas five proteins (A1-A5) were found only in mutant strains cultured at 37ºC. Furthermore, expression of five proteins (DS1-DS5) was increased according to the downshift of growth temperature to 37ºC. Among all 16 proteins, abundant three proteins (P1, DS3, and DS5) were electroblotted to PVDF membrane, followed by analyzed N-terminal amino acid sequences. As a result, it was suggested that P1 must be Surface-layer protein A (sbsA), whereas DS3 and DS5 were unknown proteins. |
| キーワード |
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|
主題 |
Thermophile |
| キーワード |
|
|
主題 |
Adaptation |
| キーワード |
|
|
主題 |
Expression |
| キーワード |
|
|
主題 |
Growth temperature |
| キーワード |
|
|
主題 |
Geobacillus stearothermophilus |
| 書誌情報 |
ja : 徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
巻 23,
号 3,
p. 87-95,
発行日 2009-10
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| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
09146385 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN10065859 |
| EID |
|
|
識別子 |
194636 |
| 言語 |
|
|
言語 |
jpn |
LID201104191009.pdf (1.17 MB)
