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Changes in Response Behaviors to Noxious Heat and Mechanical Stimuli After Carrageenan-induced Inflammation in Mice Treated with Capsaicin 2 or 15 days After Birth
https://tokushima-u.repo.nii.ac.jp/records/2002045
https://tokushima-u.repo.nii.ac.jp/records/20020458b77da82-f71e-4d41-a7a3-9da0cecbc2f1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
LID201212131001.pdf (269 KB)
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| Item type | 文献 / Documents(1) | |||||
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| 公開日 | 2012-12-17 | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 出版社版DOI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.webmedcentral.com/article_view/3727 | |||||
| 言語 | ja | |||||
| 関連名称 | 10.9754/journal.wmc.2012.003727 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| タイトル | ||||||
| タイトル | Changes in Response Behaviors to Noxious Heat and Mechanical Stimuli After Carrageenan-induced Inflammation in Mice Treated with Capsaicin 2 or 15 days After Birth | |||||
| 言語 | en | |||||
| 著者 |
樋浦, 明夫
× 樋浦, 明夫× 中川, 弘 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The aim of the present study was to investigate the cause of normal response behaviors to noxious heat despite the marked loss of small neurons in the dorsal root ganglion (DRG) as a result of neonatal capsaicin treatment. Capsaicin (50 mg/kg) was injected subcutaneously (s.c.) into mice on either day P2 or P15; control mice received a vehicle injection. Twenty days after the capsaicin injection, 2% carrageenan (20 μL) was injected into the right hind paw of each animal. Twenty-four hours after the carrageenan injection, behavioral tests using noxious heat stimuli (NHS; Hargreaves method) and noxious mechanical stimuli (NMS; von Frey method) were performed using the control and capsaicin-treated mice. Pre-carrageenan measurements were used as the baseline values for each group. After the experiments, the mice were perfused with a mixture of 4% paraformaldehyde and 0.2% picric acid in a 0.1-M phosphate buffer (pH 7.4). The L4 and L5 DRGs were extracted, sectioned using a cryostat, and immunostained using a TRPV1 antibody and reacted with isolectin IB4. The P2 capsaicin-injected mice exhibited a marked increase in their analgesic responses to NHS, compared with both their baseline values and the respective control mice. Both the capsaicin-treated and control animals exhibited significant hyperalgesia in response to NMS. Naturally, the control P15 mice exhibited a shorter response time to NHS than their baseline values, while the capsaicin-injected P15 mice exhibited increased algesia comparable to the baseline values of the control. The capsaicin-injected mice also exhibited algesia in response to NMS. The number of TRPV1-immunoreactive (ir) small neurons decreased by 90% in the P2 capsaicin-injected mice and 45% in the P15 capsaicin-injected mice, whereas an increase in IB4-positive neurons was seen in both the P2 and P15 capsaicin-injected mice. In association with the decrease in larger neurons, the numbers of smaller neurons were increased in both P2 and P15 capsaicin-injected mice. TRPV1-ir small neurons are closely correlated with inflammatory heat pain perception, suggesting the enhancement of an unknown acute noxious heat sensor in the present study. The present findings indicate that noxious mechanical stimuli can be sensed despite the presence or absence of TRPV1-ir or IB4-positive neurons. | |||||
| 言語 | en | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Noxious heat | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Noxious mechanical stimulus | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Withdrawal response | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Inflammatory pain | |||||
| 書誌情報 |
en : WebmedCentral NEUROSCIENCES 巻 3, 号 12, p. WMC003727, 発行日 2012-12-06 |
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| 収録物ID | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 20461690 | |||||
| 出版者 | ||||||
| 出版者 | WebmedCentral | |||||
| 言語 | en | |||||
| 備考 | ||||||
| 言語 | ja | |||||
| 値 | Article URL: http://www.webmedcentral.com/article_view/3727 | |||||
| 権利情報 | ||||||
| 言語 | en | |||||
| 権利情報 | This is an open-access article distributed under the terms of the Creative Commons Attribution License(CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||
| EID | ||||||
| 識別子 | 258814 | |||||
| 識別子タイプ | URI | |||||
| 言語 | ||||||
| 言語 | eng | |||||
