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Hypertrophic Chondrocytes in the Rabbit Growth Plate Can Proliferate and Differentiate into Osteogenic Cells when Capillary Invasion Is Interposed by a Membrane Filter
https://tokushima-u.repo.nii.ac.jp/records/2002478
https://tokushima-u.repo.nii.ac.jp/records/2002478bce21347-92bc-48ca-8935-5c1bd81f74aa
| 名前 / ファイル | ライセンス | アクション |
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LID201411041001.pdf (152 KB)
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LID201411041002.pdf (13.4 MB)
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| Item type | 文献 / Documents(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-11-04 | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| 出版社版DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1371/journal.pone.0104638 | |||||
| 言語 | ja | |||||
| 関連名称 | 10.1371/journal.pone.0104638 | |||||
| 出版タイプ | ||||||
| 出版タイプ | NA | |||||
| 出版タイプResource | http://purl.org/coar/version/c_be7fb7dd8ff6fe43 | |||||
| タイトル | ||||||
| タイトル | Hypertrophic Chondrocytes in the Rabbit Growth Plate Can Proliferate and Differentiate into Osteogenic Cells when Capillary Invasion Is Interposed by a Membrane Filter | |||||
| 言語 | en | |||||
| タイトル別表記 | ||||||
| その他のタイトル | 成長軟骨板において膜フィルターで血管侵入を阻害すると肥大軟骨細胞は増殖し骨芽細胞様細胞に分化する。 | |||||
| 言語 | ja | |||||
| タイトル別表記 | ||||||
| その他のタイトル | Hypertrophic Chondrocytes Differentiate into Osteogenic Cells | |||||
| 言語 | en | |||||
| 著者 |
江西, 哲也
× 江西, 哲也× 油形, 公則× 高橋, 光彦× 佐藤, 亮祐× 西良, 浩一× 安井, 夏生 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The fate of hypertrophic chondrocytes during endochondral ossification remains controversial. It has long been thought that the calcified cartilage is invaded by blood vessels and that new bone is deposited on the surface of the eroded cartilage by newly arrived cells. The present study was designed to determine whether hypertrophic chondrocytes were destined to die or could survive to participate in new bone formation. In a rabbit experiment, a membrane filter with a pore size of 1 mm was inserted in the middle of the hypertrophic zone of the distal growth plate of ulna. In 33 of 37 animals, vascular invasion was successfully interposed by the membrane filter. During 8 days, the cartilage growth plate was enlarged, making the thickness 3-fold greater than that of the nonoperated control side. Histological examination demonstrated that the hypertrophic zone was exclusively elongated. At the terminal end of the growth plate, hypertrophic chondrocytes extruded from their territorial matrix into the open cavity on the surface of the membrane filter. The progenies of hypertrophic chondrocytes (PHCs) were PCNA positive and caspase-3 negative. In situ hybridization studies demonstrated that PHCs did not express cartilage matrix proteins anymore but expressed bone matrix proteins. Immunohistochemical studies also emonstrated that the new matrix produced by PHCs contained type I collagen, osteonectin, and osteocalcin. Based on these results, we concluded that hypertrophic chondrocytes switched into bone-forming cells after vascular invasion was interposed in the normal growth plate. | |||||
| 言語 | en | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| 主題 | 軟骨細胞 | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| 主題 | 成長軟骨版 | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| 主題 | 軟骨内骨化 | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| 主題 | 骨芽細胞 | |||||
| キーワード | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| 主題 | 分化 | |||||
| 書誌情報 |
en : PLOS ONE 巻 9, 号 8, p. e104638, 発行日 2014-08-14 |
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| 収録物ID | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 19326203 | |||||
| 出版者 | ||||||
| 出版者 | PLOS | |||||
| 言語 | en | |||||
| 備考 | ||||||
| 言語 | ja | |||||
| 値 | 内容要旨・審査要旨・論文本文の公開: 内容要旨・審査要旨 : LID201411041001.pdf 論文本文 : LID201411041002.pdf 本論文は, 著者Tetsuya Enishiの学位論文として提出され, 学位審査・授与の対象となっている。 学位授与者所属 : 徳島大学大学院医科学教育部（医学専攻） |
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| 権利情報 | ||||||
| 言語 | en | |||||
| 権利情報 | Copyright: © 2014 Enishi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||
| EID | ||||||
| 識別子 | 285827 | |||||
| 識別子タイプ | URI | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 報告番号 | ||||||
| 学位授与番号 | 甲第2778号 | |||||
| 学位記番号 | ||||||
| 言語 | ja | |||||
| 値 | 甲医第1223号 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2014-10-23 | |||||
| 学位名 | ||||||
| 言語 | ja | |||||
| 学位名 | 博士（医学） | |||||
| 学位授与機関 | ||||||
| 言語 | ja | |||||
| 学位授与機関名 | 徳島大学 | |||||
