| Item type |
文献 / Documents(1) |
| 公開日 |
2016-02-29 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
http://www.jci.org/articles/view/118986/version/1/pdf/render |
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|
関連名称 |
10.1172/JCI118986 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Prevention of Adoptively Transferred Diabetes in Nonobese Diabetic Mice with IL-10–Transduced Islet-specific Th1 Lymphocytes A Gene Therapy Model for Autoimmune Diabetes |
| 著者 |
モリタニ, マキ
吉本, 勝彦
イイ, セツコ
コンドウ, マキ
イワハナ, ヒロユキ
ヤマオカ, タカシ
佐野, 壽昭
ナカノ, ナオコ
キクタニ, ヒトシ
板倉, 光夫
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| 抄録 |
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|
内容記述 |
Four pancreatic islet-specific CD4+ helper T (Th) 1 (Th1) clones and two Th1 clones transduced with an SRα promoter-linked murine IL-10 (mIL-10) cDNA of 2.0–6.0×10[6] cells were adoptively transferred to nonobese diabetic (NOD) mice at age 8 d. Cyclophosphamide (CY) was administered at age 37 d (plus CY), and the incidence of diabetes and the histological grade of insulitis were examined at age 47 d. After the adoptive transfer of IL-10–transduced Th1 cells, polymerase chain reaction (PCR) and reversetranscription (RT)-PCR detected the neo gene and the retrovirus vector-mediated IL-10 mRNA in situ in recipient islets, respectively. RT-PCR detected the decrease of IFN-γ mRNA relative to IL-10 mRNA in IL-10–transduced Th1 clones in vitro and also in recipient islets. All four wild type Th1 clones plus CY induced the insulitis grade of 2.75 and diabetes in 66% of recipient NOD mice. IL-10–transduced two Th1 clones plus CY induced periinsulitis with the grade of 1.43 and diabetes in 8.0%. The 1:1 mixture of wild type Th1 cells and IL-10–transduced Th1 cells plus CY induced periinsulitis with the grade of 1.85 and diabetes in 20%. The suppression of diabetes through decreasing IFN-γ mRNA by the tissue-specific delivery of IL-10 to pancreatic islets with IL-10–transduced Th1 cells affords us the starting basis to develop the gene therapy for autoimmune diabetes. |
| キーワード |
|
|
主題 |
insulin-dependent diabetes mellitus |
| キーワード |
|
|
主題 |
insulitis |
| キーワード |
|
|
主題 |
Th2 |
| キーワード |
|
|
主題 |
pancreatic islet B cells (islet B-cells) |
| キーワード |
|
|
主題 |
somatic gene therapy |
| 書誌情報 |
en : Journal of Clinical Investigation
巻 98,
号 8,
p. 1851-1859,
発行日 1996-10-15
|
| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
00219738 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00695520 |
| 権利情報 |
|
|
権利情報 |
© The American Society for Clinical Investigation, Inc. |
| EID |
|
|
識別子 |
71487 |
| 言語 |
|
|
言語 |
eng |
LID201602262007.pdf (310 KB)
