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蛋白質リン酸化と脱リン酸化による骨形成と骨吸収
https://tokushima-u.repo.nii.ac.jp/records/2003046
https://tokushima-u.repo.nii.ac.jp/records/20030469e739bea-5c7b-4355-8493-370a6f11fbc3
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
LID201608242006.pdf (2.43 MB)
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| Item type | 文献 / Documents(1) | |||||||||||
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| 公開日 | 2016-08-24 | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | open access | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| 出版タイプ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
| タイトル | ||||||||||||
| タイトル | 蛋白質リン酸化と脱リン酸化による骨形成と骨吸収 | |||||||||||
| タイトル | ||||||||||||
| タイトル | タンパクシツ リンサンカ ト ダツリンサンカ ニヨル コツケイセイ ト コツキュウシュウ | |||||||||||
| タイトル別表記 | ||||||||||||
| その他のタイトル | Protein Kinases and Protein Phosphatases in Bone Formation and Bone Resorption | |||||||||||
| 著者 |
羽地, 達次
× 羽地, 達次
WEKO
167
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| 抄録 | ||||||||||||
| 内容記述 | Serine and threonine protein phosphatases (PP) are divided into four categories, PP1, PP2A, PP2B, and PP2C. cDNA cloning revealed the existence of at least four isoforms of PP1 catalytic subunit in rat, termed PP1α, PP1γ1, PP1γ2, and PP1δ. Aanti-PP1δ antibody recognized a protein present in the nucleolar regions in human and mouse osteoblasts. Cellular fractionation revealed that PP1δ is a 37 kDa protein localized in the nucleolus. C23 (nucleolin) is a nucleolar phosphoprotein and located mainly in the nucleolus. Staining pattern of C23 in human osteoblasts was similar to that of the PP1δ. PP1δ and C23 were specifically stained as dots in the nucleus. The dual fluorescence images revealed that PP1δ and C23 were localized in the same regions in the nucleolus. These results indicate that PP1δ associate with C23 directly in the nucleolus and suggest that C23 is one of the candidates of PP1δ substrates. Double-stranded RNA-dependent protein kinase (PKR) is a serine/threonine protein kinase expressed in mammalian cells. PKR is activated by double-stranded RNA (dsRNA), interferon, cytokines, stress signals, and viral infections. Dominant-negative mutant PKR cDNA, in which the amino acid lysine at 296 was replaced with arginine and which does not have catalytic activity, was constructed. The mutant cDNA was transfected into mouse osteoblastic MC3T3-E1 cells and preosteoclastic mouse macrophage RAW264.7 cells. Using these cells, our group demonstrated that PKR plays important roles in the differentiation and calcification of osteoblasts by modulating STAT1α and/or Runx2 expression. In this review, I also described that PKR is required for the osteoclast formation of preosteoclastic cell by fusing these cells to form multi-nucleated giant cells. |
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| キーワード | ||||||||||||
| 主題 | PP1δ | |||||||||||
| キーワード | ||||||||||||
| 主題 | PKR | |||||||||||
| キーワード | ||||||||||||
| 主題 | C23 | |||||||||||
| キーワード | ||||||||||||
| 主題 | 骨芽細胞 | |||||||||||
| キーワード | ||||||||||||
| 主題 | 破骨細胞 | |||||||||||
| 書誌情報 |
ja : 四国歯学会雑誌 巻 24, 号 2, p. 45-50, 発行日 2012-01-31 |
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| 収録物ID | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 09146091 | |||||||||||
| 収録物ID | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AN10050046 | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | 四国歯学会 | |||||||||||
| EID | ||||||||||||
| 識別子 | 267471 | |||||||||||
| 言語 | ||||||||||||
| 言語 | jpn | |||||||||||
