| Item type |
文献 / Documents(1) |
| 公開日 |
2017-06-09 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1038/oncsis.2016.18 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Ultraconserved region-containing Transformer 2β4 controls senescence of colon cancer cells |
|
言語 |
en |
| 著者 |
Kajita, K
桑野, 由紀
Satake, Y
Kano, S
Kurokawa, K
Akaike, Y
増田, 清士
西田, 憲生
六反, 一仁
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Ultraconserved regions (UCRs) are 4200 bp genomic segments with perfect human-to-rodent sequence identity. Transcribed UCRs constitute a new category of noncoding RNAs whose functions remain poorly understood. The human transformer 2β (TRA2B) gene contains a 419-bp UCR spanning the 276-bp exon 2 and its neighboring introns. TRA2B exon 2 has premature stop codons, whereas an exon 2-containing splice variant (TRA2β4) was expressed preferentially in the nuclei of human colon cancer cells. TRA2β4 knockdown p53-independently stimulated CDKN1A transcription and increased p21, resulting in the appearance of senescent cells. Biotin pull-down and RNA immunoprecipitation assays revealed that TRA2β4 interacted with Sp1 through a Sp1-binding sequence (485-GGGG-488) in a stem-loop structure of exon 2. Mutation of this sequence (485-AAGG-488) disrupted the stem-loop structure, blocked the interaction with Sp1 and increased CDKN1A transcription. Overexpression of TRA2β4 significantly decreased CDKN1A mRNA levels and accelerated cell growth, but the introduction of the mutation in the Sp1-binding sequence completely canceled these effects. Taken together, TRA2β4 may sequester Sp1 from occupying promoters of target genes including CDKN1A, promoting cell growth by interrupting the senescence-related gene expression program. This novel function of TRA2β4 may uncover an oncogenic function of transcribed UCRs. |
|
言語 |
en |
| 書誌情報 |
en : Oncogenesis
巻 5,
p. e213,
発行日 2016-04-04
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
21579024 |
| 備考 |
|
|
言語 |
ja |
|
値 |
Oncogenesis is an open-access journal published by Nature Publishing Group. |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| EID |
|
|
識別子 |
311940 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
LID201706024005.pdf (2.31 MB)
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