| アイテムタイプ |
文献 / Documents(1) |
| 公開日 |
2017-06-09 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.2152/jmi.63.219 |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Serine/arginine-rich splicing factor 7 regulates p21-dependent growth arrest in colon cancer cells |
| 著者 |
サイジョウ, サキ
桑野, 由紀
増田, 清士
WEKO
272
e-Rad_Researcher
00457318
| ja |
増田, 清士
増田, 清士
ISNI
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| ja-Kana |
マスダ, キヨシ
マスダ, キヨシ
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| en |
Masuda, Kiyoshi
Masuda, Kiyoshi
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Search repository
西川, 達哉
六反, 一仁
西田, 憲生
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| 抄録 |
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内容記述 |
Serine/arginine-rich splicing factors (SRSFs) play wide-ranging roles in gene expression through post-transcriptional regulation as well as pre-mRNA splicing. SRSF7 was highly expressed in colon cancer tissues, and its knockdown inhibited cell growth in colon cancer cells (HCT116) in association with altered expression of 4,499 genes. The Ingenuity Pathway Analysis revealed that cell cycle-related canonical pathways were ranked as the highly enriched category in the affected genes. Western blotting confirmed that p21, a master regulator in cell cycle, was increased without any induction of p53 in SRSF7 knockdown cells. Furthermore, cyclin-dependent kinase 2 and retinoblastoma protein were remained in the hypophosphorylated state. In addition, the SRSF7 knockdown-induced cell growth inhibition was observed in p53-null HCT116 cells, suggesting that p53-independent pathways were involved in the SRSF7 knockdown-induced cell growth inhibition. The reduction of SRSF7 stabilized cyclin-dependent kinase inhibitor 1A (CDKN1A) mRNA without any activation of the CDKN1A promoter. Interestingly, SRSF7 knockdown also blocked p21 degradation. These results suggest that the reduction of SRSF7 post-transcriptionally regulates p21 induction at the multistep processes. Thus, the present findings disclose a novel, important role of SRSF7 in cell proliferation through regulating p21 levels. |
| キーワード |
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主題 |
SRSF7 |
| キーワード |
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主題 |
cell cycle |
| キーワード |
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主題 |
p21 |
| キーワード |
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主題 |
G1/S arrest |
| 書誌情報 |
en : The journal of medical investigation : JMI
巻 63,
号 3-4,
p. 219-226,
発行日 2016-08
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
13431420 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA11166929 |
| EID |
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識別子 |
331561 |
| 言語 |
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言語 |
eng |
LID201706024006.pdf (513 KB)
