| Item type |
文献 / Documents(1) |
| 公開日 |
2017-11-15 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_1843 |
|
資源タイプ |
other |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
嗅球におけるステロイド合成酵素の局在 |
|
言語 |
ja |
| タイトル |
|
|
タイトル |
キュウキュウ ニオケル ステロイド ゴウセイ コウソ ノ キョクザイ |
|
言語 |
ja-Kana |
| タイトル別表記 |
|
|
その他のタイトル |
Localization of steroid-synthesizing enzymes in the olfactory bulb |
|
言語 |
en |
| 著者 |
清蔭, 恵美
樋田, 一徳
山本, 登志子
石村, 和敬
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Neurosteroids are synthesized through mechanism at least partly independent of the peripheral steroidogenic glands, and their neurobiological actions seem to depend on the specific functions of various brain regions. However, little is known about neurosteroids function corresponding to specific structure and functions of the brain regions. Thus the present study analyzed localization of steroid-synthesizing enzymes in the rat and mouse olfactory bulb. RT-PCR and Western-blotting indicated the possible presence of almost all enzymes of steroid synthesis from cholesterol to estradiol, that is, cholesterol side chain cleavage enzyme (P450scc : cholesterol→pregnenolone), 3β-hydroxysteroid dehydrogenase/Δ5 - 4 isomerase (3β-HSD : pregnenolone→progesterone), 17α-hydroxylase/C17-C20lyase (P450c17 : progesterone→ androstenedione), 17β-hydroxysteroid dehydrogenase (17β-HSD : androstenedione→testosterone), 5α-reductase (5αR:testosterone→5α-dihydrotestosterone), and aromatase (testosterone→ estradiol). Immunohistochemistry confirmed that the 5α-reductase was mainly in glial cells with various immunoreactivity and co-localization pattern, but other enzymes were in bulbar neurons, and at least co-localized in mitral/tufted cells. In addition, so far analyzed, enzymatic activities of P450c17 (a key enzyme in sex steroid synthesis) and 17β-HSD were detected biochemically. These enzyme expression and activities were influenced under exposure of 17β-estradiol with various degrees. These findings indicated the presence of steroid-synthesizing activities, and thus of possible neurosteroid metabolism in the olfactory bulb. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
neurosteroid |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
olfactory bulb |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
enzyme |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
steroid |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
immunohistochemistry |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
localization |
| 書誌情報 |
ja : 四国医学雑誌
巻 59,
号 4-5,
p. 250-251,
発行日 2003-10-25
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
00373699 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00102041 |
| 出版者 |
|
|
出版者 |
徳島医学会 |
|
言語 |
ja |
| EID |
|
|
識別子 |
136932 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
jpn |
sam_59_4-5_250.pdf (19.7 KB)
