| Item type |
文献 / Documents(1) |
| 公開日 |
2017-11-22 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版タイプ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Effect of human airway trypsin-like protease on intracellular free Ca2+ concentration in human bronchial epithelial cells |
| 著者 |
ミキ, マリ
ナカムラ, ヨウイチ
髙橋, 章
中屋, 豊
エグチ, ヒロシ
マセギ, ツキオ
ヨネダ, カズオ
安岡, 劭
曽根, 三郎
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| 抄録 |
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内容記述 |
It has been shown that human airway trypsin-like protease (HAT) is localized in human bronchial epithelial cells (HBEC), and trypsin activates protease-activated receptor-2(PAR-2). Activation of PAR-2 activates G-protein followed by an increase of intracellular free Ca2+, [Ca2+]in. This study was undertaken to clarify whether HAT can activate PAR-2in HBEC or not. RT-PCR showed that HAT mRNA is expressed in HBEC, and PAR-2 mRNA is the most strongly expressed of the known PARs in HBEC. Both PAR-2 agonist peptide (PAR-2 AP) and HAT increased [Ca2+]in in HBEC in a biphasic fashion a prompt, sharp increase (peak I) and a sustained low plateau (peak II). PAR-2 AP over 100-200 μM and HAT over 200-300 mU/ml (0.08-0.12 μM) induced both peak I and II, and PAR-2 AP below100 μM and HAT below 200 mU/ml induced only peak II. Both PAR-2 AP-induced and HAT-induced peak I were induced by Ca2+ mobilization from intracellular stores, because they appeared even in Ca2+-free medium. Both PAR-2 AP-induced and HAT-induced peak II were induced by an influx of extracellular Ca2+, because they were abolished in Ca2+-free medium. The Ca2+ response to HAT was desensitized by exposure of HBEC to PAR-2 AP. These results indicate that HBEC have a functional PAR-2, and HAT regulates cellular functions of HBEC via activation of PAR-2. |
| キーワード |
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主題 |
human airway trypsin-like protease (HAT) |
| キーワード |
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|
主題 |
human bronchial epithelial cells (HBEC) |
| キーワード |
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|
主題 |
protease-activated receptor-2(PAR-2) |
| キーワード |
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|
主題 |
intracellular calcium |
| 書誌情報 |
en : The journal of medical investigation : JMI
巻 50,
号 1-2,
p. 95-107,
発行日 2003
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13431420 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11166929 |
| EID |
|
|
識別子 |
146488 |
| 言語 |
|
|
言語 |
eng |
jmi_50_1-2_95.pdf (164 KB)
