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Medical significance of cysteine protease inhibitors in mammalian secretory fluids
https://tokushima-u.repo.nii.ac.jp/records/2003888
https://tokushima-u.repo.nii.ac.jp/records/20038883a4f7297-1e34-4565-a4c9-12db1ab83d32
| 名前 / ファイル | ライセンス | アクション |
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jmi_50_3-4_154.pdf (158 KB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||
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| 公開日 | 2017-11-20 | |||||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||
| タイトル | ||||||||||||||||||||
| タイトル | Medical significance of cysteine protease inhibitors in mammalian secretory fluids | |||||||||||||||||||
| 著者 |
カツヌマ, ノブヒコ
× カツヌマ, ノブヒコ
× 塩田, 洋
WEKO
1523
× Le, Quang Trong
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| 抄録 | ||||||||||||||||||||
| 内容記述 | New cysteine protease inhibitors in human tears and milk and their medical significance are reviewed in this paper. As protective components against bacterial infection in the eyes, we detected four kinds of anti-bacterial proteins in normal human tears including lysozyme and three kinds of cysteine protease inhibitors. Using our reverse zymography of normal tears, three kinds of cysteine protease inhibitors were found to be 78kDa, 20kDa and 15kDa and were determined to be lactoferrin, Von Ebner’s Gland(VEG)protein and cystatin S, respectively. All of them belong to the cystatin super family and VEG protein and cystatin S are well known cysteine protease inhibitors. TheC-terminusarea17mer peptide,Y679-K695,of lactoferrin showed strong homology with a common active domain of the cystatin family and the synthesized peptide showed inhibition of cysteine proteases. Not only were disease-specific changes found in these inhibitor profiles, but also disease-specific new inhibitors in patients tear with certain autoimmune diseases. A 35kDa inhibitor, which was detected specifically in tears with Behcet’s disease, an typical autoimmune disease, was determined to be a lacrimal acidic proline-rich protein based on the N-terminus sequence analysis. A65kDa inhibitor of tears with Harada’s autoimmune disease was determined to be an Ig heavy chain V-III region. In addition, lactoferrin content in Harada’s disease was very low. We found two cathepsin inhibitors in bovine milk using reverse zymography, namely lactoferrinandβ-casein. TheL133-Q151, in the humanβ-casein moleculeis the active inhibitory domain. They may play an important role in antiseptic and anti-infectious functions. | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 主題 | cystein protease inhibitor | |||||||||||||||||||
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| 主題 | human tears | |||||||||||||||||||
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| 主題 | reverse zymography | |||||||||||||||||||
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| 主題 | Behcet’s disease | |||||||||||||||||||
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| 主題 | lactoferrin | |||||||||||||||||||
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| 主題 | milk | |||||||||||||||||||
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| 主題 | autoimmune disease | |||||||||||||||||||
| 書誌情報 |
en : The journal of medical investigation : JMI 巻 50, 号 3-4, p. 154-161, 発行日 2003 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||
| 収録物識別子 | 13431420 | |||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||
| 収録物識別子 | AA11166929 | |||||||||||||||||||
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| 識別子 | 133709 | |||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||
