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Inhibitory effect of FK506 and Cyclosporine A on the growth and invasion of human liver cancer cells
https://tokushima-u.repo.nii.ac.jp/records/2003913
https://tokushima-u.repo.nii.ac.jp/records/2003913fdf05799-c559-47e0-a075-b65f2c7ba47c
| 名前 / ファイル | ライセンス | アクション |
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jmi_51_1-2_63.pdf (266 KB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||
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| 公開日 | 2017-11-22 | |||||||||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.2152/jmi.51.63 | |||||||||||||||||||||||
| 関連名称 | 10.2152/jmi.51.63 | |||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||
| タイトル | Inhibitory effect of FK506 and Cyclosporine A on the growth and invasion of human liver cancer cells | |||||||||||||||||||||||
| 著者 |
サカイ, マナブ
× サカイ, マナブ
× 三宅, 秀則
× 田代, 征記× 奥村, 裕司× 木戸, 博
WEKO
173
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| 内容記述 | Background : The prognosis of liver transplantation for liver cancer is determined by recurrence in the liver graft. In this study, the effects of immunosuppressors, FK506 and cyclosporine A (CsA) on the migration of liver cancer cells were investigated. Methods : The effects of FK506 at concentrations of 1-100 ng/mL and CsA at 1-1000ng/mL on the growth of poorly and well differentiated human hepatocellular carcinoma cell lines, HLE and HuH-7, respectively, were examined. After treatment of these cells with FK506 and CsA, the growth of these cells, their cytotoxicities and invasion assay on the Matrigel basement membrane invasion chamber were evaluated. In addition, the effects of FK506 and CsA on the changes in the production of a soluble intercellular adhesionmolecule-1 (sICAM-1) of these cells were measured. Results : FK506 and CsA at concentrations of 1-10 ng/mL inhibited the growth of both HLE and HuH-7 and those immunosuppressors at concentrations over 100 ng/mL exhibited cytotoxicity on these cells. FK506 at concentration of 1ng/mL significantly inhibited the invasion of poorly differentiated HLE, but not well differentiated HuH-7, after treatment for 2-5 days in culture (p＜0.05), but FK 506 at 10 ng/mL showed less inhibitory efficient. CsA at concentrations of 1-10 ng/mL, however, did not inhibit or transiently inhibited the invasion of both cell lines. The production of ICAM-1 in HLE and HuH-7 was suppressed by FK506 at concentrations of 1-10 ng/mL after treatment for 3-5 days but the effect was not significant in the initial phase at days 1-2 and the last phase at days 5-6. Conclusions : FK506, but not CsA, at a clinical dose of 1ng/mL significantly inhibited the invasion of the poorly-differentiated HLE, but not HuH-7 and also inhibited the production of sICAM-1 in HLE. | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | FK 506 | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | cyclosporine A | |||||||||||||||||||||||
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| 主題 | liver cancer cell | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | invasion | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | ICAM-1 | |||||||||||||||||||||||
| 書誌情報 |
en : The journal of medical investigation : JMI 巻 51, 号 1-2, p. 63-69, 発行日 2004-02 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||
| 収録物識別子 | 13431420 | |||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||
| 収録物識別子 | AA11166929 | |||||||||||||||||||||||
| EID | ||||||||||||||||||||||||
| 識別子 | 154907 | |||||||||||||||||||||||
| 言語 | ||||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||||
