| Item type |
文献 / Documents(1) |
| 公開日 |
2017-11-28 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
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|
関連識別子 |
https://doi.org/10.2152/jmi.53.1 |
|
|
関連名称 |
10.2152/jmi.53.1 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Principle and clinical usefulness of the infrared fluorescence endoscopy |
| 著者 |
伊東, 進
六車, 直樹
木村, 哲夫
矢野, 弘美
イモト, ヨシタカ
岡本, 耕一
カジ, マサコ
佐野, 茂樹
長尾, 善光
|
| 抄録 |
|
|
内容記述 |
Since there is no infrared fluorescence materials in the living body, infrared fluorescence labeling materials are very useful for making a diagnosis of a micro cancer. We have developed an infrared fluorescence endoscope (IRFE) and indocyanin green (ICG)-derivative as infrared fluorescence labeling materials to evaluate gastrointestinal neoplastic lesions. The study aims were to apply an IRFE and to demonstrate its usefulness in detecting cancerous tissue using an antibody coupled with ICG-derivative. IRFE consisted of an infrared endoscope equipped with excitation (710-790nm) and barrier (810-920nm) filters and an intensified CCD camera. We have developed ICG N-hydroxy sulfo succinimide ester (ICG-sulfo-OSu) and 3-ICG-acyl-1, 3-thiazolidine-2-thione (ICG-ATT) as an infrared fluorescent-labeling reagent. ICG-derivative-labeled mouse anti-human carcinoembryonic antigen (CEA)antibodyandMUC1 antibody were employed in this study. Moreover, we examined the ability of a reinforcement agent, octylglucoside, to intensity fluorescence from the labeled antibody. Biopsy specimens of gastric cancer were stained with anti-CEA antibody by the avidin-biotinylated peroxidase complex method. Among the positive specimens, freshly resected stomach from three cases were used for the infrared (IR) imaging analysis. The incubation of freshly resected stomach specimens with ICG-anti-CEA antibody-complex resulted in positive staining of the tumor sites by IRFE, and the IR fluorescent images correlated well with the tumor sites. The immunohistochemical studies suggested that the intensity of IR fluorescence of ICG-ATT-MUC1was stronger than that of ICG-sulfo-OSu. In tumor sections, the reinforcement agent intensified fluorescence, ever at low antibody concentrations. Therefore, we conclude that an anti-CEA (and/orMUC1) antibody with affinity for cancerous lesions and labeled with ICG-derivative can be imaged with this IRFE. Specific antibodies tagged with ICG-derivative with the reinforcement agent can label cancer cells and generate a strong enough fluorescent signal to detect small cancers when examined with an IR fluorescence endoscope. |
| キーワード |
|
|
主題 |
infrared fluorescence |
| キーワード |
|
|
主題 |
infrared fluorescence endoscopy |
| キーワード |
|
|
主題 |
indocyanin green delivative |
| キーワード |
|
|
主題 |
endoscopic diagnosis |
| キーワード |
|
|
主題 |
bioendscopy |
| 書誌情報 |
en : The journal of medical investigation : JMI
巻 53,
号 1-2,
p. 1-8,
発行日 2006-02
|
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13431420 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11166929 |
| EID |
|
|
識別子 |
164034 |
| 言語 |
|
|
言語 |
eng |
jmi_53_1-2_1.pdf (259 KB)
