| Item type |
文献 / Documents(1) |
| 公開日 |
2017-11-28 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.2152/jmi.53.52 |
|
|
言語 |
ja |
|
|
関連名称 |
10.2152/jmi.53.52 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Optimal labeling condition of antibodies available for immunofluorescence endoscopy |
|
言語 |
en |
| 著者 |
タダツ, ヨウコ
六車, 直樹
伊東, 進
タダツ, マサヤ
クサカ, ヨシヒロ
岡本, 耕一
イモト, ヨシタカ
タウエ, ヒロミ
佐野, 茂樹
長尾, 善光
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Purpose: In recent years, labeled antibodies have been used for diagnostic imaging in many studies. In this study, we investigated the mode of binding in antibodies labeled with ICG derivatives newly developed for the diagnosis of microcarcinomas, and evaluated the optimal binding molar ratio between the labeling compounds and antibody. Methods: MUC1 antibody and ICG derivatives (ICG-ATT and ICG-sulfo-OSu) were used. ICG derivatives noncovalently bound to the antibody were removed with ethyl acetate, and the ratio of ICG derivatives covalently bound to the labeled antibody was confirmed. During purification of the labeled antibody, the amount of each labeling compound reacting with 1molecule of the antibody varied as follows: 4, 8, 16, and 32molar equivalents. Subsequently, the intensity of fluorescence was evaluated by spectroscopy and infrared fluoroscopy. Results : The ratio of residual ICG derivative labeling the antibody was 67.4% for ICG-ATT and 65.0% for ICG-sulfo-OSu. When fluorescent antibody labeled with ICG-ATT at anF/Pratio of 2.94or 4.18wasused, specific and clear fluorescent images of the antigen were obtained. When ICG-ATT-labeled antibody at an F/P ratio of 6.50 or 6.75 was used, the fluorescence intensity decreased and the fluorescent images of antigen became unclear. Conclusions: It was found that the ICG-ATT-labeled antibody was a more specific and sensitive marker than ICG-sulfo-OSu-labeled antibody, and that lower binding molar ratios of ICG-ATT were more useful for labeling the antibody. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
immunofluorescence |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
infrared fluorescence |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
labeled antibody |
| 書誌情報 |
en : The journal of medical investigation : JMI
巻 53,
号 1-2,
p. 52-60,
発行日 2006-02
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| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13431420 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11166929 |
| EID |
|
|
識別子 |
164031 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
jmi_53_1-2_52.pdf (219 KB)
