| Item type |
文献 / Documents(1) |
| 公開日 |
2017-12-07 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
A rapid and simple system of detecting deletions on the Y chromosome related with male infertility using multiplex PCR |
|
言語 |
en |
| 著者 |
梅野, 真由美
新家, 利一
佐藤, 陽一
Yang, Xin-Jun
馬場, 嘉信
イワモト, テルアキ
中堀, 豊
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Around 10% of males with idiopathic azoospermia or oligozoospermia, which are important causes of male infertility, have partial deletions on the long arm of the Y chromosome. To develop a rapid and accurate detection system for screening major Y deletions found in infertile men, we developed a multiplex PCR system that can simultaneously amplify five loci on the Y chromosome, SRY, AMELY, DBY, RBMY, DAZ and one locus on the X chromosome, AMELX. The size of the PCR products was designed to increase gradually from the distal Yp to the distal Yq. Our system could detect deletions of three major candidate regions for the azoospermic factor, AZFa, AZFb and AZFc on the Y chromosome together with sex. The gradual increase in the size of the PCR products was convenient for imaging the location of deletions on the Y chromosome. Moreover, the multiplex PCR system was combined with microchip-based electrophoresis, and the PCR products derived from each locus were separated within 4min. Our system is useful for screening Y chromosomes bearing the structural anomalies including three major AZF deletions found among azoospermic or oligozoospermic males. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Y chromosome |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
AZF |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
multiplex PCR |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
microchip electrophoresis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
azoospermia |
| 書誌情報 |
en : The journal of medical investigation : JMI
巻 53,
号 1-2,
p. 147-152,
発行日 2006-02
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13431420 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11166929 |
| EID |
|
|
識別子 |
160577 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
jmi_53_1-2_147.pdf (122 KB)
