| Item type |
文献 / Documents(1) |
| 公開日 |
2017-12-07 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.2152/jmi.53.264 |
|
|
言語 |
ja |
|
|
関連名称 |
10.2152/jmi.53.264 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Histochemical and immunohistochemical investigation of guanase and nedasin in human tissues |
|
言語 |
en |
| 著者 |
クボ, ケンイチロウ
本田, 浩仁
サンノミヤ, カツタカ
Aying, Yuan
Mei, Wei
Mi, Shen
アオヤギ, エリコ
清水, 一郎
イイ, クニオ
伊東, 進
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Guanase is known as an enzyme released from the liver. Recently, cloning and sequencing of the guanase gene were reported. In addition, almost simultaneously, it was reported that an unknown protein that binds to neuronal and endocrine lethal (1)-discs large (NE-dlg), one of the membrane-associated guanylate kinase homologues (MAGUK) family proteins involved in synaptic connection between neurons, was cloned and named nedasin (NE-dlg associated protein), whose sequence was almost identical to that of guanase. We immunostained fresh frozen sections of surgically removed human liver, kidney, and small intestine with anti-nedasin antibody, and simultaneously performed histochemical staining for guanase for comparison. Histochemically, guanase activity was observed in the cytoplasm of hepatocytes and biliary epitheliumon the liver, in the mucosal epithelium on the small intestine, and in the proximal tubule on the kidney. Immunohistochemically, a brown discoloration due to DAB oxidation was seen in the cytoplasm of hepatocytes and biliary epithelium on the liver, in the proximal tubule but in the distal tubule a little on the kidney, in the mucosal epithelium on the small intestine. The stained region of the liver and the small intestine were different from that of the kidney. The different staining properties dependent on the organs were considered to be due to different isozymes. The physiological significance of guanase may vary with the isozymes, further studies are considered necessary. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
guanase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
nedasin |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
histochemical staining |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
immunohistochemical staining |
| 書誌情報 |
en : The journal of medical investigation : JMI
巻 53,
号 3-4,
p. 264-270,
発行日 2006-08
|
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13431420 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11166929 |
| EID |
|
|
識別子 |
147900 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
jmi_53_3-4_264.pdf (240 KB)
