| Item type |
文献 / Documents(1) |
| 公開日 |
2017-11-20 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
BMP2-induced gene profiling in dental epithelial cell line |
|
言語 |
en |
| 著者 |
三好, 圭子
ナガタ, ヒデヤ
堀口, 大吾
阿部, 佳織
Wahyudi, Ivan Arie
馬場, 嘉信
ハラダ, ヒデミツ
野間, 隆文
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Tooth development is regulated by epithelial-mesenchymal interactions and their reciprocal molecular signaling. Bone morphogenetic protein 2 (BMP2) is known as one of the inducers for tooth development. To analyze the molecular mechanisms of BMP2 on ameloblast differentiation (amelogenesis), we performed microarray analyses using rat dental epithelial cell line, HAT-7. After confirming that BMP2 could activate the canonical BMP-Smads signaling in HAT-7 cells, we analyzed the effects of BMP2 on 14,815 gene expressions and profiled them. Seventy-three genes were up-regulated and 28 genes were down-regulated by BMP2 treatment for 24 hours in HAT-7 cells. Functional classification revealed that 18% of up-regulated genes were ECM/adhesion molecules present in the enamel organ. Furthermore, we examined the expression of several differentiation markers in dental epithelial four cell-lineages including inner enamel epithelium (ameloblasts), stratum intermedium, stratum reticulum, and outer enamel epithelium. The results indicated that BMP2 might induce at least two different cell-lineage markers including a BMP antagonist expressed in HAT-7 cells, suggesting that BMP2 could accelerate amelogenesis via BMP signaling. |
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言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
dental epithelium |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
BMP2 |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
microarray |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
gene profiling |
| 書誌情報 |
en : The journal of medical investigation : JMI
巻 55,
号 3-4,
p. 216-226,
発行日 2008-08
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| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13431420 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11166929 |
| EID |
|
|
識別子 |
191545 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
jmi_55_3-4_216.pdf (817 KB)
