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Effect of exposure to an extremely low frequency- electromagnetic field on the cellular collagen with respect to signaling pathways in osteoblast-like cells
https://tokushima-u.repo.nii.ac.jp/records/2004067
https://tokushima-u.repo.nii.ac.jp/records/2004067683d3512-0666-46a6-ad39-be03ba660a28
| 名前 / ファイル | ライセンス | アクション |
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jmi_55_3-4_267.pdf (854 KB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||||||||
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| 公開日 | 2017-11-20 | |||||||||||||||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.2152/jmi.55.267 | |||||||||||||||||||||||||||||
| 関連名称 | 10.2152/jmi.55.267 | |||||||||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||||||||
| タイトル | Effect of exposure to an extremely low frequency- electromagnetic field on the cellular collagen with respect to signaling pathways in osteoblast-like cells | |||||||||||||||||||||||||||||
| 著者 |
荘田, 彰
× 荘田, 彰
× 池原, 敏孝
× 木内, 陽介
WEKO
82
× 吉﨑, 和男
WEKO
290
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| 内容記述 | The effect of exposure to extremely low frequency-electromagnetic field (ELFEMF : 3 mT, 60 Hz) on differentiation of mouse osteoblast-like MC3T3-E1 cells was examined together with addition of insulin-like growth factor I (IGF-I). As a marker of the differentiation, the cellular collagen content was determined by the absorbance of Sirius red-stained cells measured at the wavelength of 510-520 nm with an imaging microspectroscopy. Exposure to ELF-EMF increased significantly the collagen in the cells. Treatment with PD98059, an inhibitor of extracellular signal-regulated kinase 1/2 (ERK1/2) activation, reduced the collagen in all of the cells examined on control, IGF-I addition and ELFEMF exposure, however, PD98059 did not prevent the increase in the collagen caused by ELF-EMF exposure, and IGF-I also increased the collagen in the presence of the inhibitor. When phosphatidylinositol 3-kinase (PI3K) pathway was inhibited by LY294002, the increase in collagen induced by ELF-EMF exposure was accelerated, however, the increase in collagen observed by IGF-I addition was suppressed. Treatment with SB203580, an inhibitor of p38 mitogen-activated protein kinase (p38 MAPK), suppressed the increase in the collagen induced by ELF-EMF exposure, whereas IGF-I addition increased the collagen in the presence of the inhibitor. These results suggested that collagen synthesis stimulated by ELF-EMF exposure was carried out by the participation of p38 MAPK pathway, and that PI3K pathway may have the role to suppress the collagen synthesis induced by ELF-EMF exposure, and that the suppression of the PI3K pathway may allow the acceleration of the collagen synthesis. | |||||||||||||||||||||||||||||
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| 主題 | ELF-EMF | |||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||
| 主題 | ERK1/2 | |||||||||||||||||||||||||||||
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| 主題 | PI3K | |||||||||||||||||||||||||||||
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| 主題 | p38 MAPK | |||||||||||||||||||||||||||||
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| 主題 | collagen | |||||||||||||||||||||||||||||
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| 主題 | osteoblast | |||||||||||||||||||||||||||||
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| 主題 | MC3T3-E1 cell | |||||||||||||||||||||||||||||
| 書誌情報 |
en : The journal of medical investigation : JMI 巻 55, 号 3-4, p. 267-278, 発行日 2008-08 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||||||||
| 収録物識別子 | 13431420 | |||||||||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||||||||
| 収録物識別子 | AA11166929 | |||||||||||||||||||||||||||||
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| 識別子 | 178841 | |||||||||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||||||||
