| Item type |
文献 / Documents(1) |
| 公開日 |
2017-11-28 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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|
関連識別子 |
https://doi.org/10.2152/jmi.56.26 |
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関連名称 |
10.2152/jmi.56.26 |
| 出版タイプ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
Polyphenols prevent clinorotation-induced expression of atrogenes in mouse C2C12 skeletal myotubes |
| 著者 |
Hemdan, Dalia Ismaeil Ibrahim
平坂, 勝也
中尾, 玲子
コウノ, ショウヘイ
カガワ, サチコ
安倍, 知紀
原田, 晃子
奥村, 裕司
中屋, 豊
寺尾, 純二
二川, 健
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| 抄録 |
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内容記述 |
Oxidative stress is a key factor in stimulating the expression of atrogenes, which are muscle atrophy-related ubiquitin ligases, in skeletal muscle, and it induces muscle atrophy during unloading. However, the effects of antioxidative nutrients on atrogene expression have not been demonstrated. We report on the inhibitory effects of polyphenols, such as epicatechin (EC), epicatechin gallate (ECg) and epigallocatechin gallate (EGCg) and quercetin, on atrogene expression up-regulated by three dimensional (3D)-clinorotation or glucocorticoid. These treatments markedly elevated the expression of atrogenes, including atrogin-1 and MuRF-1, in mouse C2C12 myoblasts and myotubes. Interestingly, EC, ECg, EGCg and quercetin significantly decreased the expression of atrogin-1 and MuRF-1 up-regulated by 3D-clinorotation, whereas they hardly affected atrogene expression induced by dexamethasone. ERK signaling is a well known MAPK pathway to mediate oxidative stress. Therefore, we also investigated the effect of these polyphenols on phosphorylation of ERK in C2C12 myotubes. As expected, EC, ECg, EGCg, and quercetin significantly suppressed phosphorylation of ERK, corresponding to the up-regulation of atrogenes induced by 3D-clinorotation. These results suggest that antioxidative nutrients, such as catechins and quercetin, suppress atrogene expression in skeletal muscle cells, possibly through the inhibition of ERK signaling. Thus, catechins and quercetin may prevent unloading-mediated muscle atrophy. |
| キーワード |
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主題 |
atrogenes |
| キーワード |
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主題 |
dexamethasone |
| キーワード |
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|
主題 |
mouse C2C12 cells |
| キーワード |
|
|
主題 |
polyphenols |
| キーワード |
|
|
主題 |
3D-clinorotation |
| 書誌情報 |
en : The journal of medical investigation : JMI
巻 56,
号 1-2,
p. 26-32,
発行日 2009-02
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
13431420 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11166929 |
| EID |
|
|
識別子 |
292318 |
| 言語 |
|
|
言語 |
eng |
jmi_56_1-2_26.pdf (554 KB)
