| Item type |
文献 / Documents(1) |
| 公開日 |
2017-11-22 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
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|
関連識別子 |
https://doi.org/10.1016/j.ijpharm.2016.11.042 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
|
|
タイトル |
Development of flexible nanocarriers for siRNA delivery into tumor tissue |
| 著者 |
Jung, Hyunkyung
シマタニ, ユウリ
Hasan, Mahadi
ウノ, コウヘイ
ハマ, ススム
小暮, 健太朗
|
| 抄録 |
|
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内容記述 |
Various non-viral delivery systems for small interfering RNAs (siRNA) have been developed. Such delivery systems generally exhibit tightly formed spherical structures. While such carriers have demonstrated good transfection activity in mono-layered cell systems, effects against solid tumors are often less apparent and difficult to demonstrate, likely due to the rigid structures of the carriers, which may prevent penetration to deeper regions within tumor tissue. Herein, we developed a flexible nanocarrier (FNC) system that is able to penetrate to deeper regions within tumor tissue. Specifically, we employed previously found flexible polyplexes comprised of siRNA and poly-L-lysine as wick structures for the preparation of FNCs. FNCs were constructed by coating the wick structures with lipids using a liposomal membrane fusion method. The diameters of the resulting FNCs were ca. 170 nm, and the shapes were non-spherical. Lipid coating was confirmed using a nuclease resistance assay. Furthermore, FNCs showed significant RNA interference effects, comparable to Lipofectamine 2000, in a mono-layered cell system. To accelerate tumor penetration, the FNC surface was modified with polyethylene glycol (PEG) and the tight junction opener peptide AT1002. Surface-modified FNCs demonstrated effective penetrability into a cancer spheroid. Thus, we developed a novel and unique tumor-penetrable siRNA FNC system. |
| キーワード |
|
|
主題 |
flexible nanocarrier |
| キーワード |
|
|
主題 |
intercellular penetrability |
| キーワード |
|
|
主題 |
siRNA/PLL polyplexes |
| 書誌情報 |
en : International Journal of Pharmaceutics
巻 516,
号 1-2,
p. 258-265,
発行日 2016-11-18
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
03785173 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00680771 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11530828 |
| 出版者 |
|
|
出版者 |
Elsevier |
| 権利情報 |
|
|
権利情報 |
© 2016 Elsevier B.V. All rights reserved.
© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| EID |
|
|
識別子 |
321618 |
| 言語 |
|
|
言語 |
eng |
ijpharm_516_1-2_258.pdf (1.31 MB)
