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Effects of Co-Transfection with Myostatin-Targeting siRNA and ActRIIB-Fc Fusion Protein on Skeletal Muscle Growth
https://tokushima-u.repo.nii.ac.jp/records/2004252
https://tokushima-u.repo.nii.ac.jp/records/2004252a291f44b-6b7b-44ce-9040-9f4118c5cc60
| 名前 / ファイル | ライセンス | アクション |
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LID201704281013.pdf (74.4 KB)
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LID201704281014.pdf (34.6 KB)
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k3085_fulltext.pdf (1.01 MB)
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| Item type | 文献 / Documents(1) | |||||||||||
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| 公開日 | 2018-02-09 | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | open access | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||||
| 資源タイプ | doctoral thesis | |||||||||||
| 出版社版DOI | ||||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.20738/johb.30.1_1 | |||||||||||
| 言語 | ja | |||||||||||
| 関連名称 | 10.20738/johb.30.1_1 | |||||||||||
| 出版タイプ | ||||||||||||
| 出版タイプ | NA | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_be7fb7dd8ff6fe43 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Effects of Co-Transfection with Myostatin-Targeting siRNA and ActRIIB-Fc Fusion Protein on Skeletal Muscle Growth | |||||||||||
| 言語 | en | |||||||||||
| タイトル別表記 | ||||||||||||
| その他のタイトル | Myostatin-siRNAおよびActivinIIB型受容体融合タンパク質の共導入による骨格筋形成制御効果の検討 | |||||||||||
| 言語 | ja | |||||||||||
| 著者 |
バイヤルサイハナ, オド
× バイヤルサイハナ, オド
× 川合, 暢彦× 森, 博世× 木内, 奈央× 二川, 健× 田中, 栄二 |
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Background: Myostatin (Mstn) is a secreted TGF-β family member that controls skeletal muscle growth, and binds with high affinity to the activin type IIB receptor (ActRIIB). The soluble ligand-binding domain of ActRIIB fused to the Fc domain of IgG (ActRIIB-Fc) potently binds and inhibits TGF-β family members in muscle, leading to rapid and marked muscle growth. The present study was designed to assess the combinative effects of myostatin-targeting siRNA (Mstn-siRNA) and ActRIIB-Fc on murine myoblast in vitro and in vivo. Materials and Methods: C2C12 cells were treated by Mstn-siRNA with or without ActRIIB-Fc at 0 and 48 h after differentiation. Myotube size was measured, and gene expression of Mstn, MuRF-1, MyoD and myogenin were analyzed. Furthermore, 11-week-old, male C57BL/6 mice were injected with atelocollagen (ATCOL)-mediated Mstn-siRNA and Mstn-siRNA/ActRIIB-Fc locally into the masseter muscle twice a week. Histological and biochemical analyses were performed using the dissected muscles. Results: Transfection of Mstn-siRNA and Mstn-siRNA/ActRIIB-Fc resulted in significant increases in the myotube diameter of the C2C12 cells compared with untreated control. Also, treatment with Mstn-siRNA and Mstn-siRNA/ActRIIB-Fc could lead to an upregulation of MyoD and myogenin gene expression and downregulation of Mstn and MuRF-1. In vivo, muscle fibril hypertrophy was observed in both Mstn-siRNA and Mstn-siRNA/ActRIIB-Fc treated groups. Moreover, western blotting analysis showed that the p-Smad2/3 expression level was decreased by treatment of Mstn-siRNA/ActRIIB-Fc. In contrast, MyoD and myogenin protein levels were increased by combined treatment, compared with the other groups. Conclusions: These suggest that double inhibition of myostain is potentially useful for myogenesis and muscle growth promotion. This may be a good as new treatment remedy for patients with various muscle atrophies, including muscular dystrophy. |
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| 言語 | en | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | myostatin | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | small-interfering RNAs | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | activin type IIB receptor | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | muscle hypertrophy | |||||||||||
| 書誌情報 |
en : Journal of Oral Health and Biosciences 巻 30, 号 1, p. 1-7, 発行日 2017-06-30 |
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| 収録物ID | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 21896682 | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | 四国歯学会 | |||||||||||
| 言語 | ja | |||||||||||
| 備考 | ||||||||||||
| 言語 | ja | |||||||||||
| 値 | 内容要旨・審査要旨・論文本文の公開： 内容要旨：LID201704281013.pdf 審査要旨：LID201704281014.pdf 論文本文：k3085_fulltext.pdf 本論文は，著者Od BAYARSAIKHANの学位論文として提出され，学位審査・授与の対象となっている。 学位授与者所属 : 徳島大学大学院口腔科学教育部（口腔科学専攻） |
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| EID | ||||||||||||
| 識別子 | 338805 | |||||||||||
| 識別子タイプ | URI | |||||||||||
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| 言語 | eng | |||||||||||
| 報告番号 | ||||||||||||
| 学位授与番号 | 甲第3085号 | |||||||||||
| 学位記番号 | ||||||||||||
| 言語 | ja | |||||||||||
| 値 | 甲口第428号 | |||||||||||
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| 学位授与年月日 | 2017-03-23 | |||||||||||
| 学位名 | ||||||||||||
| 言語 | ja | |||||||||||
| 学位名 | 博士（歯学） | |||||||||||
| 学位授与機関 | ||||||||||||
| 言語 | ja | |||||||||||
| 学位授与機関名 | 徳島大学 | |||||||||||
