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Generation and characterization of new CCR5-tropic HIV-1rmt clones
https://tokushima-u.repo.nii.ac.jp/records/2004320
https://tokushima-u.repo.nii.ac.jp/records/2004320ee78f453-1548-4409-ba1f-34dea5fc529d
| 名前 / ファイル | ライセンス | アクション |
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jmi_64_3-4_272.pdf (662 KB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||
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| 公開日 | 2018-03-08 | |||||||||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.2152/jmi.64.272 | |||||||||||||||||||||||
| 関連名称 | 10.2152/jmi.64.272 | |||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||
| タイトル | Generation and characterization of new CCR5-tropic HIV-1rmt clones | |||||||||||||||||||||||
| 著者 |
土肥, 直哉
× 土肥, 直哉× サカイ, ヨウスケ
× 足立, 昭夫
WEKO
1594
× 野間口, 雅子
WEKO
246
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| 内容記述 | To develop effective non-human primate models for coping with numerous HIV-1/AIDS studies, rhesus macaque-tropic HIV-1 (HIV-1rmt) clones with a variety of biological properties are required. Such clones, if available, are powerful tools to experimentally elucidate HIV-1 replication and pathogenicity in host individuals, and also to develop anti-HIV-1 drugs/vaccines. However, only limited numbers of HIV-1rmt clones have been currently reported. In the present study, we generated new HIV-1rmt clones carrying various CCR5-tropic env (envelope) genes by standard recombinant DNA and intracellular homologous recombination techniques. Resultant virus clones contain the env sequences derived from an AIDS-inducible laboratory or two clinically isolated viral strains.We further constructed their variant clones bearing N160K, S304G, or G310R mutation in Env that potentially can change the viruses to better grow. Newly generated clones were analyzed for their virological properties such as Env expression, single-cycle infectivity, and multi-cycle replication ability. Out of a number of new clones examined, two were found to grow better in macaque cells than the previously constructed clone used for comparison. Our study described here constitutes the initial and essential step towards obtaining CCR5-tropic HIV-1rmt clones useful for various basic and clinical research projects on infected individuals. | |||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||
| 主題 | HIV-1rmt | |||||||||||||||||||||||
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| 主題 | CCR5-tropic | |||||||||||||||||||||||
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| 主題 | Env | |||||||||||||||||||||||
| 書誌情報 |
en : The Journal of Medical Investigation 巻 64, 号 3-4, p. 272-279, 発行日 2017-08 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||
| 収録物識別子 | 13496867 | |||||||||||||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||
| 収録物識別子 | 13431420 | |||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||
| 収録物識別子 | AA11166929 | |||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||
| 収録物識別子 | AA12022913 | |||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||
| 出版者 | Faculty of Medicine Tokushima University | |||||||||||||||||||||||
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| 識別子 | 341521 | |||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||
