| アイテムタイプ |
文献 / Documents(1) |
| 公開日 |
2018-06-04 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.2152/jmi.56.241 |
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関連名称 |
10.2152/jmi.56.241 |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Salivary gland development : its mediation by a subtilisinlike proprotein convertase, PACE4 |
| 著者 |
赤松, 徹也
Azlina, Ahmad
Javkhlan, Purevjav
長谷川, 敬展
姚, 陳娟
細井, 和雄
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| 抄録 |
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内容記述 |
The submandibular gland (SMG) develops under the epithelial-mesenchymal interaction. Its process is regulated by various growth/differentiation factors, which are synthesized as inactive precursors and activated via the limited proteolysis at their multi basic amino acid site(s) such as Arg-X-Lys/Arg-Arg. Although many of these processing steps are elucidated to be catalyzed by subtilisin-like proprotein convertases (SPCs), little is known about the role of SPCs in the SMG development. Here, we focused upon the physiological role of PACE4 (SPC4), a member of SPC family, in the SMG development. In the organ culture system of rat embryonic SMG (E15), Dec-RVKR-CMK, a potent inhibitor for SPCs, inhibited the salivary branching and the expression of an exocrine gland type water channel, AQP5. However, other peptidyl-CMKs and inhibitors for trypsin-like serine proteases including leupeptin did not affect the salivary branching and AQP5 expression. Dec-RVKR-CMK also suppressed the expression of PACE4, but not furin, another member of the family. The specific antibody for the catalytic domain of PACE4 suppressed the salivary branching and AQP5 expression similarly. These inhibitory effects of Dec-RVKR-CMK were partially rescued by the addition of recombinant BMP2 whose precursor is a candidate for the physiological substrates of PACE4. Further, the transcriptional silencing of PACE4 by its specific siRNAs caused the suppression of both the salivary branching and AQP5 expression in the present organ culture system. These observations strongly support the idea that PACE4 mediates the SMG development. |
| キーワード |
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主題 |
subtilisin-like proprotein convertase |
| キーワード |
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主題 |
PACE4 |
| キーワード |
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主題 |
branching morphogenesis |
| キーワード |
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主題 |
water channel |
| キーワード |
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主題 |
AQP5 |
| キーワード |
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主題 |
SMG |
| キーワード |
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主題 |
development |
| 書誌情報 |
en : The Journal of Medical Investigation
巻 56,
号 Supplement,
p. 241-246,
発行日 2009-12
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
13496867 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
13431420 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA11166929 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA12022913 |
| 出版者 |
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出版者 |
Faculty of Medicine Tokushima University |
| EID |
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識別子 |
213995 |
| 言語 |
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言語 |
eng |
jmi_56_suppl_241.pdf (317 KB)
