| アイテムタイプ |
文献 / Documents(1) |
| 公開日 |
2018-06-07 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.2152/jmi.56.273 |
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関連名称 |
10.2152/jmi.56.273 |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Down-regulation of submandibular gland AQP5 following parasympathetic denervation in rats |
| 著者 |
Azlina, Ahmad
李, 雪飛
Javkhlan, Purevjav
長谷川, 敬展
姚, 陳娟
赤松, 徹也
細井, 和雄
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| 抄録 |
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内容記述 |
Following chorda tympani denervation (CTD, parasympathetomy), the protein levels of aquaporin5 (AQP5) as well as AQP1 and Na+K+ATPase α-subunit in the rat submandibular gland (SMG) were found to be decreased significantly. However, the level of another membrane protein, dipeptidyl peptidase IV was not affected by CTD, suggesting a selective reduction of AQP5, AQP1, and Na+K+ATPase1α-subunit proteins by CTD. However, the AQP5 mRNA level was scarcely affected by CTD, which suggested that transcription process of AQP5 was unaffected by this operation. AQP5 protein was shown to be degraded in vitro by the extract of the SMG obtained from normal rat ; inhibitor experiments in vitro suggested cathepsin B was a responsible enzyme. Co-localization of AQP5 and LAMP-2, a lysosomal marker, implicated AQP5 is degraded in lysosomes. A significant increase in the protein levels of LC3-II, an autophagy marker, at day 1 after CTD, and co-localization of the LC3 protein and AQP5, suggested that CTD activated autophagy of SMG, leading to AQP5 degradation. |
| キーワード |
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主題 |
chorda tympani denervation |
| キーワード |
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主題 |
AQP5 |
| キーワード |
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主題 |
degradation |
| キーワード |
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主題 |
autophagy |
| 書誌情報 |
en : The Journal of Medical Investigation
巻 56,
号 Supplement,
p. 273-276,
発行日 2009-12
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
13496867 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
13431420 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA11166929 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA12022913 |
| 出版者 |
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出版者 |
Faculty of Medicine Tokushima University |
| EID |
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|
識別子 |
213998 |
| 言語 |
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言語 |
eng |
jmi_56_suppl_273.pdf (1.43 MB)
