| Item type |
文献 / Documents(1) |
| 公開日 |
2018-06-07 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.2152/jmi.56.287 |
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関連名称 |
10.2152/jmi.56.287 |
| 出版タイプ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Induction of calprotectin mRNAs by lipopolysaccharide in the salivary gland of mice |
| 著者 |
Javkhlan, Purevjav
廣島, 佑香
Azlina, Ahmad
長谷川, 敬展
姚, 陳娟
赤松, 徹也
木戸, 淳一
永田, 俊彦
細井, 和雄
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| 抄録 |
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内容記述 |
Calprotectin is a major cytosolic calcium-binding protein of leukocytes which belongs to the S100 protein family. S100A8 and S100A9, major types of calprotectin are heterodimeric complexes being composed of light- and heavy-chain subunits. The calprotectin levels in the plasma, feces, synovial fluid, gingival crevicular fluid, dental calculus and saliva change when the host animal suffers from several inflammatory diseases. Members of Toll-like receptor (TLR) family are pattern-recognition receptors for lipopolysaccharide (LPS) and other pathogens. Here we examined if the biological role of TLR receptor is reflected to the calprotectin expression in the salivary gland. Time course study by using real-time RT-PCR detected higher levels of S100A8 and S100A9 mRNA at 1.5-3 h after injection of LPS in both the submandibular gland (SMG) and parotid gland (PG) of C3H/HeN mice but not in the same tissues of C3H/HeJ, a TLR-4 mutant strain, indicating that this induction is mediated via the TLR-4. These results indicate that, an inflammatory marker, calprotectin, is expressed in the mouse salivary gland and that LPS stimulated its synthesis. Calprotectin (S100A8/A9) showed minimum expression in all cellular segments in the SMG except excretory duct cells, which showed strong signal at the cytoplasm. LPS induced their expressions in the granular convoluted tubular cells and striated duct cells. In the PG, these proteins were expressed very weakly in both duct and acinar cells with a little stronger staining for the former cells. LPS injection induced calprotectin (S100A8/A9) in both duct and acinar cells especially in the former cells. |
| キーワード |
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主題 |
Calprotectin |
| キーワード |
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主題 |
LPS |
| キーワード |
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|
主題 |
salivary gland |
| 書誌情報 |
en : The Journal of Medical Investigation
巻 56,
号 Supplement,
p. 287-289,
発行日 2009-12
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
13496867 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13431420 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11166929 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12022913 |
| 出版者 |
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|
出版者 |
Faculty of Medicine Tokushima University |
| EID |
|
|
識別子 |
213999 |
| 言語 |
|
|
言語 |
eng |
jmi_56_suppl_287.pdf (58.2 KB)
