| アイテムタイプ |
文献 / Documents(1) |
| 公開日 |
2018-04-25 |
| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1016/j.cyto.2017.04.017 |
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関連名称 |
10.1016/j.cyto.2017.04.017 |
| 出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
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タイトル |
The AMPK/mTOR pathway is involved in D-dopachrome tautomerase gene transcription in adipocytes differentiated from SGBS cells, a human preadipocyte cell line |
| 著者 |
岩田, 武男
クリバヤシ, キョウコ
ナカソノ, マサヒコ
田良島, 典子
南川, 典昭
水澤, 典子
木戸, 理恵
吉本, 勝彦
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| 抄録 |
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内容記述 |
In adipose tissue, D-dopachrome tautomerase (DDT), a cytokine with structural similarity to macrophage migration inhibitory factor, is mainly expressed in adipocytes rather than preadipocytes and acts as an anti-obesity adipokine in an autocrine manner. However, its transcriptional regulation is largely unknown. In order to explore molecules affecting DDT transcription, a chemical library screening using HEK293 cells stably expressing a DDT promoter-reporter construct was performed. Several derivatives of 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside (AICAR), an AMP-activated protein kinase (AMPK) activator, were identified as transcriptional activators of the DDT gene. Furthermore, DDT mRNA levels were reduced in SGBS adipocytes treated with compound C, an AMPK inhibitor, suggesting involvement of AMPK in DDT transcription. Overexpression of the FOXO1 constitutive active form reduced transcriptional activity of the DDT gene in SGBS cells, but increased it in HEK293 cells. Cell-type specific effects were also observed in the DDT gene expression of cells treated with AS1842856, a FOXO1 inhibitor. Finally, involvement of the mammalian target of rapamycin (mTOR) signaling in DDT transcription in SGBS adipocytes was investigated. Rapamycin, an inhibitor of mTOR, increased DDT mRNA levels and attenuated the inhibitory effects of compound C on DDT mRNA levels in SGBS adipocytes. In conclusion, DDT transcription may be regulated in a cell-dependent manner, and were enhanced by AMPK activation in SGBS adipocytes through inhibiting the mTOR signaling. |
| キーワード |
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主題 |
D-dopachrome tautomerase |
| キーワード |
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主題 |
adipose tissue |
| キーワード |
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|
主題 |
AMPK |
| キーワード |
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|
主題 |
FOXO1 |
| キーワード |
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主題 |
mTOR |
| 書誌情報 |
en : Cytokine
巻 96,
p. 195-202,
発行日 2017-04-25
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
10434666 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA10794201 |
| 出版者 |
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出版者 |
Elsevier |
| 権利情報 |
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|
権利情報 |
© 2017 Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| EID |
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|
識別子 |
325960 |
| 言語 |
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|
言語 |
eng |
cyto_96_195.pdf (679 KB)
