| Item type |
文献 / Documents(1) |
| 公開日 |
2018-07-03 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_1843 |
|
資源タイプ |
other |
| 出版社版DOI |
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|
識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.2152/jmi.56.376 |
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言語 |
ja |
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|
関連名称 |
10.2152/jmi.56.376 |
| 出版タイプ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
Ca2+ mobilization by nicotine through synaptic activation in rat parotid acini |
|
言語 |
en |
| 著者 |
イイダ, タイチ
オノ, ケンタロウ
イナガキ, トモヒロ
ホソカワ, リュウジ
イネナガ, キヨトシ
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Nicotine has been reported to increase the intracellular Ca2+ concentration ([Ca2+]i) in sublingual acini due to neurotransmitter release from nerve terminals associated with the cell preparation (1). However, it is unclear whether or not the same reaction exists in parotid cells. Therefore, we investigated effect of nicotine on Ca2+ mobilization in digested parotid acini from rats. After removing the parotid gland from Wistar rats, the tissues were minced and digested with collagenase. Then, the intracellular Ca2+ indicator fura-2 was added to the preparation, and the change in [Ca2+]i was monitored using fluorescent microscope. In many but not all parotid acini, K+ stimulation induced transient increases in [Ca2+]i. The K+-induced Ca2+ response in parotid acini was completely blocked by Cd2+-containing solution. These results suggest that the parotid cell preparation has nerve terminals. In all high-K+-sensitive parotid acini, over 3μM of nicotine increased [Ca2+]i, and the response was blocked by a Cd2+-containing solution and nicotinic receptor antagonists. All high-K+-insensitive acinar cells were resistant to the effect of nicotine on Ca2+ mobilization. These results suggest that nicotine induces increases in [Ca2+]i in parotid acini due to neurotransmitter release from associated nerve terminals. |
|
言語 |
en |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
nicotine |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
nerve terminal |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
parotid cells |
| 書誌情報 |
en : The Journal of Medical Investigation
巻 56,
号 Supplement,
p. 376-376,
発行日 2009-12
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
13496867 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13431420 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11166929 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12022913 |
| 出版者 |
|
|
出版者 |
Faculty of Medicine Tokushima University |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
jmi_56_suppl_376.pdf (38.5 KB)
