| Item type |
文献 / Documents(1) |
| 公開日 |
2018-06-06 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.2152/jmi.54.276 |
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関連名称 |
10.2152/jmi.54.276 |
| 出版タイプ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Characterization of follistatin-related gene as a negative regulatory factor for activin family members during mouse heart development |
| 著者 |
タケハラ カサマツ, ユカ
ツチダ, クニヒロ
ナカタニ, マサシ
ムラカミ, タツヤ
クリサキ, アキラ
ハシモト, オサム
大内, 淑代
クロセ, ヒトミ
モリ, カズヒロ
香美, 祥二
野地, 澄晴
スギノ, ヒロム
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| 抄録 |
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内容記述 |
Follistatin-related gene (FLRG) encodes a secretory glycoprotein that has characteristic cysteine-rich follistatin domains. FLRG protein binds to and neutralizes several transforming growth factor-β (TGF-β) superfamily members, including myostatin (MSTN), which is a potent negative regulator of skeletal muscle mass. We have previously reported that FLRG was abundantly expressed in fetal and adult mouse heart. In this study, we analyzed the expression of FLRG mRNA during mouse heart development. FLRG mRNA was continuously expressed in the embryonic heart, whereas it was very low in skeletal muscles. By contrast, MSTN mRNA was highly expressed in embryonic skeletal muscles, whereas the expression of MSTN mRNA was rather low in the heart. In situ hybridization and immunohistochemical analysis revealed that FLRG expressed in smooth muscle of the aorta and pulmonary artery, valve leaflets of mitral and tricuspid valves, and cardiac muscles in the ventricle of mouse embryonic heart. However, MSTN was expressed in very limited areas, such as valve leaflets of pulmonary and aortic valves, the top of the ventricular and atrial septa. Interestingly, the expression of MSTN was complementary to that of FLRG, especially in the valvular apparatus. Biochemical analyses with surface plasmon resonance biosensor and reporter assays demonstrated that FLRG hardly dissociates from MSTN and activin once it bound to them, and efficiently inhibits these activities. Our results suggest that FLRG could function as a negative regulator of activin family members including MSTN during heart development. |
| キーワード |
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主題 |
FLRG |
| キーワード |
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|
主題 |
myostatin |
| キーワード |
|
|
主題 |
activin |
| キーワード |
|
|
主題 |
follistatin |
| キーワード |
|
|
主題 |
heart |
| 書誌情報 |
en : The Journal of Medical Investigation
巻 54,
号 3-4,
p. 276-288,
発行日 2007-08
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
13496867 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13431420 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11166929 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12022913 |
| 出版者 |
|
|
出版者 |
Faculty of Medicine Tokushima University |
| EID |
|
|
識別子 |
174132 |
| 言語 |
|
|
言語 |
eng |
jmi_54_3-4_276.pdf (1.42 MB)
