| アイテムタイプ |
文献 / Documents(1) |
| 公開日 |
2018-05-31 |
| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1507/endocrj.50.309 |
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関連名称 |
10.1507/endocrj.50.309 |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Human Pituitary Adenomas Infrequently Contain Inactivation of Retinoblastoma 1 Gene and Activation of Cyclin Dependent Kinase 4 Gene |
| タイトル別表記 |
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その他のタイトル |
RETINOBLASTOMA GENE IN PITUITARY TUMORS |
| 著者 |
ホンダ, ソウイチ
(田中)小杉, 知里
ヤマダ, ショウゾウ
佐野, 壽昭
松本, 俊夫
板倉, 光夫
吉本, 勝彦
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| 抄録 |
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内容記述 |
Components of cyclinD1/cyclin-dependent kinase 4 (CDK4)/p16INK4a/pRb pathway are the frequent target of many tumor types. We examined the role of retinoblastoma susceptibility gene (RB1) and the CDK4 gene in human pituitary tumorigenesis. For the RB1 gene, pRb expression and loss of heterozygosity (LOH) on 13q in pituitary adenomas were analysed. Immunostaining of pRb revealed lack of expression in 1 of 29 pituitary adenomas. In 4 of 31 pituitary adenomas, allelic imbalances including LOH of RB1 on 13q14 were detected. Three of 4 pituitary adenomas, in which one adenoma lacked pRb expression, had a common LOH region at least from D13S219 on 13q12.3-q13 to D13S265 on 13q31-32. Interphase fluorescence in situ hybridization with a probe of RB1 showed 2 copies of RB1 gene suggesting that mitotic recombination events, not deletion or chromosome loss, led to LOH in the 3 pituitary adenomas analyzed. All 27 exons, intron-exon boundaries, and essential promoter region of RB1 gene were then sequenced in genomic DNA from 4 pituitary adenomas with allelic imbalance on 13q14 including one adenoma without pRb expression and 3 adenomas with pRb expression. Any somatic mutations, insertions, or microdeletions in the RB1 gene were not detected in 4 pituitary adenomas. Methylation sensitive (MS)-polymerase chain reaction (PCR) and bisulfite sequencing analysis revealed hypomethylated status of CpG islands in the promoter region of the RB1 genes of 4 pituitary adenomas. In addition, activating mutations of CDK4 gene, which is a component of cyclinD1/CDK4/p16INK4a/pRb pathway, were not detected in 31 pituitary adenomas. Based on these results, it is concluded that somatic mutations of the RB1 gene or CDK4 gene do not appear to play a major role in pituitary tumorigenesis. This supports the presence of potential tumor suppressor gene(s) on 13q12.3-q13 to 13q31-32 in pituitary adenomas. |
| キーワード |
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主題 |
Pituitary adenomas |
| キーワード |
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主題 |
RB1 |
| キーワード |
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主題 |
CDK4 |
| キーワード |
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主題 |
Mutations |
| キーワード |
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主題 |
Loss of heterozygosity |
| 書誌情報 |
en : Endocrine Journal
巻 50,
号 3,
p. 309-318,
発行日 2003
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
13484540 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
09188959 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA12020190 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA10901436 |
| 出版者 |
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出版者 |
The Japan Endocrine Society |
| EID |
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識別子 |
72276 |
| 言語 |
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言語 |
eng |
endocrj_50_3_309.pdf (2.4 MB)
