| Item type |
文献 / Documents(1) |
| 公開日 |
2018-07-03 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
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|
関連識別子 |
https://doi.org/10.1002/glia.23034 |
|
|
関連名称 |
10.1002/glia.23034 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
The Novel Monoclonal Antibody 9F5 Reveals Expression of a Fragment of GPNMB/Osteoactivin Processed by Furin-like Protease(s) in a Subpopulation of Microglia in Neonatal Rat Brain |
| 著者 |
カワハラ, コウイチ
ヒラタ, ヒロシ
オオブチ, ケンゴ
ニシ, ケンタロウ
マエダ, アキラ
クニヤス, アキヒコ
ヤマダ, ダイスケ
マエダ, タケヒコ
辻, 明彦
サワダ, マコト
ナカヤマ, ヒトシ
|
| 抄録 |
|
|
内容記述 |
To differentiate subtypes of microglia (MG), we developed a novel monoclonal antibody, 9F5, against one subtype (type 1) of rat primary MG. The 9F5 showed high selectivity for this cell type in Western blot and immunocytochemical analyses and no cross‐reaction with rat peritoneal macrophages (Mφ). We identified the antigen molecule for 9F5: the 50‐ to 70‐kDa fragments of rat glycoprotein nonmetastatic melanoma protein B (GPNMB)/osteoactivin, which started at Lys170. In addition, 9F5 immunoreactivity with GPNMB depended on the activity of furin‐like protease(s). More important, rat type 1 MG expressed the GPNMB fragments, but type 2 MG and Mφ did not, although all these cells expressed mRNA and the full‐length protein for GPNMB. These results suggest that 9F5 reactivity with MG depends greatly on cleavage of GPNMB and that type 1 MG, in contrast to type 2 MG and Mφ, may have furin‐like protease(s) for GPNMB cleavage. In neonatal rat brain, amoeboid 9F5+ MG were observed in specific brain areas including forebrain subventricular zone, corpus callosum, and retina. Double‐immunοstaining with 9F5 antibody and anti‐Iba1 antibody, which reacts with MG throughout the CNS, revealed that 9F5+ MG were a portion of Iba1+ MG, suggesting that MG subtype(s) exist in vivo. We propose that 9F5 is a useful tool to discriminate between rat type 1 MG and other subtypes of MG/Mφ and to reveal the role of the GPNMB fragments during developing brain. |
| キーワード |
|
|
主題 |
development |
| キーワード |
|
|
主題 |
GPNMB/osteoactivin |
| キーワード |
|
|
主題 |
microglial heterogeneity |
| キーワード |
|
|
主題 |
retinal pigment epithelium |
| 書誌情報 |
en : GLIA
巻 64,
号 11,
p. 1938-1961,
発行日 2016-07-27
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
08941491 |
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
10981136 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10687234 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11620396 |
| 出版者 |
|
|
出版者 |
Wiley |
| 権利情報 |
|
|
権利情報 |
© 2016 The Authors. Glia Published by Wiley Periodicals, Inc.
This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
| EID |
|
|
識別子 |
322769 |
| 言語 |
|
|
言語 |
eng |
glia_64_11_1938.pdf (2.41 MB)
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