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Identification and Characterization of a 25 kDa Protein That Is Indispensable for the Efficient Saccharification of Eisenia bicyclis in the Digestive Fluid of Aplysia kurodai
https://tokushima-u.repo.nii.ac.jp/records/2005043
https://tokushima-u.repo.nii.ac.jp/records/200504399104f7f-dfe8-4bc6-a5f9-639f88a29269
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
pone_12_1_e0170669.pdf (5.86 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||
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| 公開日 | 2018-07-03 | |||||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1371/journal.pone.0170669 | |||||||||||||||||||||||
| 言語 | ja | |||||||||||||||||||||||
| 関連名称 | 10.1371/journal.pone.0170669 | |||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||
| タイトル | Identification and Characterization of a 25 kDa Protein That Is Indispensable for the Efficient Saccharification of Eisenia bicyclis in the Digestive Fluid of Aplysia kurodai | |||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| 著者 |
辻, 明彦
× 辻, 明彦
WEKO
121
× クワムラ, シュウジ
× 白井, 昭博
WEKO
1235
× 湯浅, 恵造 |
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| 抄録 | ||||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||||
| 内容記述 | The digestive fluid of the sea hare Aplysia kurodai can liberate approximately 2.5 mg of glucose from 10 mg of dried Eisenia bicyclis powder. Although laminaran, a major storage polysaccharide in E. bicyclis, is easily digested to glucose by the synergistic action of the 110 and 210 kDa A. kurodai β-glucosidases (BGLs), glucose is not liberated from E. bicyclis by direct incubation with these BGLs. To clarify this discrepancy, we searched for an Eisenia hydrolysis enhancing protein (EHEP) in the digestive fluid of A. kurodai. A novel 25 kDa protein that enhances E. bicyclis saccharification by β-glucosidases was purified to a homogeneous state from the digestive fluid of A. kurodai, and its cDNA was cloned from total cDNAs reverse-transcribed from hepatopancreas total RNA. The E. bicyclis extract strongly inhibited BGLs, suggesting some compound within this brown alga functioned as a feeding deterrent. However, when E. bicyclis was incubated with BGLs in the presence of EHEP, glucose production was markedly increased. As E. bicyclis is rich in phlorotannin, which are only found in brown algae, our study suggested that these compounds are the main BGL inhibitors in E. bicyclis extract. EHEP protects BGLs from phlorotannin inhibition by binding to phlorotannins and forming an insoluble complex with phloroglucinol and phlorotannins. These findings indicated that EHEP plays a key role in the saccharification of brown seaweeds containing phlorotannins in the digestive fluid of A. kurodai. This is the first report of EHEP as a phlorotannin-binding protein that protects BGLs from inhibition. | |||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| 書誌情報 |
en : PLOS ONE 巻 12, 号 1, p. e0170669, 発行日 2017-01-27 |
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| 収録物ID | ||||||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||
| 収録物識別子 | 19326203 | |||||||||||||||||||||||
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| 出版者 | PLOS | |||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| 権利情報 | © 2017 Tsuji et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||||||||||||||||||||
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| 識別子 | 322766 | |||||||||||||||||||||||
| 識別子タイプ | URI | |||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||
