| Item type |
文献 / Documents(1) |
| 公開日 |
2018-07-03 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1016/j.bbrc.2017.01.001 |
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関連名称 |
10.1016/j.bbrc.2017.01.001 |
| 出版タイプ |
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|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
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|
タイトル |
Type II cGMP-dependent protein kinase negatively regulates fibroblast growth factor signaling by phosphorylating Raf-1 at serine 43 in rat chondrosarcoma cells |
| 著者 |
亀村, 典生
ムラカミ, サラ
コマツ, ヒロアキ
サワノイ, マサヒロ
ミヤモト, ケンジ
イシドウ, カズミ
岸本, 幸治
辻, 明彦
湯浅, 恵造
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| 抄録 |
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内容記述 |
Although type II cGMP-dependent protein kinase (PKGII) is a major downstream effector of cGMP in chondrocytes and attenuates the FGF receptor 3/ERK signaling pathway, its direct target proteins have not been fully explored. In the present study, we attempted to identify PKGII-targeted proteins, which are associated with the inhibition of FGF-induced MAPK activation. Although FGF2 stimulation induced the phosphorylation of ERK1/2, MEK1/2, and Raf-1 at Ser-338 in rat chondrosarcoma cells, pretreatment with a cell-permeable cGMP analog strongly inhibited their phosphorylation. On the other hand, Ser-43 of Raf-1 was phosphorylated by cGMP in a dose-dependent manner. Therefore, we examined the direct phosphorylation of Raf-1 by PKGII. Wild-type PKGII phosphorylated Raf-1 at Ser-43 in a cGMP-dependent manner, but a PKGII D412A/R415A mutant, which has a low affinity for cGMP, did not. Finally, we found that a phospho-mimic mutant, Raf-1 S43D, suppressed FGF2-induced MAPK pathway. These results suggest that PKGII counters FGF-induced MEK/ERK activation through the phosphorylation of Raf-1 at Ser-43 in chondrocytes. |
| キーワード |
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|
主題 |
PKGII |
| キーワード |
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|
主題 |
Raf-1 |
| キーワード |
|
|
主題 |
Chondrocytes |
| キーワード |
|
|
主題 |
FGF |
| 書誌情報 |
en : Biochemical and Biophysical Research Communications
巻 483,
号 1,
p. 82-87,
発行日 2017-01-03
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0006291X |
| 収録物ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00564395 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11542044 |
| 出版者 |
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|
出版者 |
Elsevier |
| 権利情報 |
|
|
権利情報 |
© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| EID |
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|
識別子 |
322767 |
| 言語 |
|
|
言語 |
eng |
bbrc_483_1_82.pdf (366 KB)
