| Item type |
文献 / Documents(1) |
| 公開日 |
2018-07-25 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.pep.2016.05.009 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1016/j.pep.2016.05.009 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
|
|
タイトル |
First characterization of extremely halophilic 2-deoxy-D-ribose-5-phosphate aldolase |
|
言語 |
en |
| 著者 |
オオシダ, タツヤ
林, 順司
サトムラ, タケノリ
川上, 竜巳
大島, 敏久
櫻庭, 春彦
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
2-Deoxy-D-ribose-5-phosphate aldolase (DERA) catalyzes the aldol reaction between two aldehydes and is thought to be a potential biocatalyst for the production of a variety of stereo-specific materials. A gene encoding DERA from the extreme halophilic archaeon, Haloarcula japonica, was overexpressed in Escherichia coli. The gene product was successfully purified, using procedures based on the protein’s halophilicity, and characterized. The expressed enzyme was stable in a buffer containing 2 M NaCl and exhibited high thermostability, retaining more than 90% of its activity after heating at 70℃ for 10 min. The enzyme was also tolerant to high concentrations of organic solvents, such as acetonitrile and dimethylsulfoxide. Moreover, H. japonica DERA was highly resistant to a high concentration of acetaldehyde and retained about 35% of its initial activity after 5-hours’ exposure to 300 mM acetaldehyde at 25℃, the conditions under which E. coli DERA is completely inactivated. The enzyme exhibited much higher activity at 25℃ than the previously characterized hyperthermophilic DERAs (Sakuraba et al., 2007). Our results suggest that the extremely halophilic DERA has high potential to serve as a biocatalyst in organic syntheses. This is the first description of the biochemical characterization of a halophilic DERA. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
halophile |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Haloarcula japonica |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
2-deoxy-D-ribose-5-phosphate aldolase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
archaea |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
organic solvent |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
aldehyde |
| 書誌情報 |
en : Protein Expression and Purification
巻 126,
p. 62-68,
発行日 2016-05-20
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
10465928 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10814149 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11542088 |
| 出版者 |
|
|
出版者 |
Elsevier |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| EID |
|
|
識別子 |
314604 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
pep_126_62.pdf (544 KB)
