| Item type |
文献 / Documents(1) |
| 公開日 |
2018-07-20 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1039/C7TX00244K |
|
|
言語 |
ja |
|
|
関連名称 |
10.1039/C7TX00244K |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
|
|
タイトル |
Cytometrical analysis of the adverse effects of indican, indoxyl, indigo, and indirubin on rat thymic lymphocytes |
|
言語 |
en |
| 著者 |
フナコシ, ユリエ
アズマ, アヤコ
イシカワ, ミヅキ
イツキ, サトル
タムラ, ヤスアキ
金丸, 芳
平井, 松午
小山, 保夫
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Many businesses thrive by producing health supplements from agricultural products, as exemplified by the production of functional (or health) food using plants traditionally cultivated in the rural areas. Dyes, such as indican, indigo, indoxyl, and indirubin, present in dye plants, possess antibacterial, antifungal, and antiproliferative activities. However, these effects may also lead to cytotoxicity. Thus, studies in normal mammalian cells are necessary to identify cytotoxicity and prevent adverse effects of functional foods that contain these dyes. In this study, the effects of indican, indigo, indoxyl, and indirubin were evaluated by flow cytometry using appropriate fluorescent probes in rat thymic lymphocytes. Among the dyes analyzed, indirubin exerted distinct cellular activities. Treatment with indirubin (10–30 μM) increased the population of shrunken dead cells. The side scatter, but not forward scatter, increased in indirubin-treated living cells. It increased the population of annexin V-bound living and dead cells and that of dead cells without annexin V. Indirubin elevated intracellular Ca2+, but not Zn2+ levels. The cellular content of superoxide anions and increased glutathione decreased. Indirubin depolarized the cellular plasma and mitochondrial membranes. It did not potentiate or attenuate the cytotoxicity of A23187 (Ca2+ overload) and H2O2 (oxidative stress). The results suggested that indirubin induces both apoptotic and non-apoptotic cell death. It may be difficult to predict and prevent adverse effects of indirubin due to its diverse activities on normal mammalian cells. Therefore, indirubin should be removed from products that contain dye plant extracts. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Indican |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Indigo |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Indoxyl |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Indirubin |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Cytotoxicity |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Lymphocytes |
| 書誌情報 |
en : Toxicology Research
巻 7,
号 3,
p. 513-520,
発行日 2018-03-28
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20454538 |
| 出版者 |
|
|
出版者 |
The Royal Society of Chemistry |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
© Royal Society of Chemistry 2018 |
| EID |
|
|
識別子 |
337162 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
toxires_7_3_513.pdf (2.89 MB)
