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A C-terminal motif contributes to the plasma membrane localization of Arabidopsis STP transporters
https://tokushima-u.repo.nii.ac.jp/records/2005496
https://tokushima-u.repo.nii.ac.jp/records/2005496e48d7c21-0706-4e14-a1d6-aa100616d02f
| 名前 / ファイル | ライセンス | アクション |
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pone_12_10_e0186326.pdf (6.85 MB)
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| Item type | 文献 / Documents(1) | |||||||||||
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| 公開日 | 2018-11-12 | |||||||||||
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| アクセス権 | open access | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| 出版社版DOI | ||||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.1371/journal.pone.0186326 | |||||||||||
| 言語 | ja | |||||||||||
| 関連名称 | 10.1371/journal.pone.0186326 | |||||||||||
| 出版タイプ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
| タイトル | ||||||||||||
| タイトル | A C-terminal motif contributes to the plasma membrane localization of Arabidopsis STP transporters | |||||||||||
| 言語 | en | |||||||||||
| タイトル別表記 | ||||||||||||
| その他のタイトル | A novel ER export signal of STP transporters | |||||||||||
| 言語 | en | |||||||||||
| 著者 |
山田, 晃嗣
× 山田, 晃嗣× 刑部, 祐里子× ヤマグチ シノザキ, カズコ
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Membrane trafficking is highly organized to maintain cellular homeostasis in any organisms. Membrane-embedded transporters are targeted to various organelles to execute appropriate partition and allocation of their substrates, such as ions or sugars. To ensure the fidelity of targeting and sorting, membrane proteins including transporters have sorting signals that specify the subcellular destination and the trafficking pathway by which the destination is to be reached. Here, we have identified a novel sorting signal (called the tri-aromatic motif) which contains three aromatic residues, two tryptophans and one histidine, for the plasma membrane localization of sugar transporters in the STP family in Arabidopsis. We firstly found that a C-terminal deletion disrupted the sugar uptake activity of STP1 in yeast cells. Additional deletion and mutation analyses demonstrated that the three aromatic residues in the C-terminus, conserved among all Arabidopsis STP transporters, were critical for sugar uptake by not only STP1 but also another STP transporter STP13. We observed that, when the tri-aromatic motif was mutated, STP1 was largely localized at the endomembrane compartments in yeast cells, indicating that this improper subcellular localization led to the loss of sugar absorption. Importantly, our further analyses uncovered that mutations of the tri-aromatic motif resulted in the endoplasmic reticulum (ER) retention of STP1 and STP13 in plant cells, suggesting that this motif is involved at the step of ER exit of STP transporters to facilitate their plasma membrane localization. Together, we here identified a novel ER export signal, and showed that appropriate sorting via the tri-aromatic motif is important for sugar absorption by STP transporters. | |||||||||||
| 言語 | en | |||||||||||
| 書誌情報 |
en : PLOS ONE 巻 12, 号 10, p. e0186326, 発行日 2017-10-13 |
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| 収録物ID | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 19326203 | |||||||||||
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| 出版者 | PLOS | |||||||||||
| 言語 | en | |||||||||||
| 権利情報 | ||||||||||||
| 言語 | en | |||||||||||
| 権利情報 | Copyright: © 2017 Yamada et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||||||||
| EID | ||||||||||||
| 識別子 | 334224 | |||||||||||
| 識別子タイプ | URI | |||||||||||
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| 言語 | eng | |||||||||||
