| Item type |
文献 / Documents(1) |
| 公開日 |
2018-11-12 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1038/srep24878 |
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言語 |
ja |
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関連名称 |
10.1038/srep24878 |
| 出版タイプ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Mechano-adaptive sensory mechanism of α-catenin under tension |
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言語 |
en |
| 著者 |
マキ, コウイチロウ
Han, Sung-Woong
ヒラノ, ヨシノリ
米村, 重信
ハコシマ, トシオ
アダチ, タイジ
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The contractile forces in individual cells drive the tissue processes, such as morphogenesis and wound healing, and maintain tissue integrity. In these processes, α-catenin molecule acts as a tension sensor at cadherin-based adherens junctions (AJs), accelerating the positive feedback of intercellular tension. Under tension, α-catenin is activated to recruit vinculin, which recruits actin filaments to AJs. In this study, we revealed how α-catenin retains its activated state while avoiding unfolding under tension. Using single-molecule force spectroscopy employing atomic force microscopy (AFM), we found that mechanically activated α-catenin fragment had higher mechanical stability than a non-activated fragment. The results of our experiments using mutated and segmented fragments showed that the key intramolecular interactions acted as a conformational switch. We also found that the conformation of α-catenin was reinforced by vinculin binding. We demonstrate that α-catenin adaptively changes its conformation under tension to a stable intermediate state, binds to vinculin, and finally settles into a more stable state reinforced by vinculin binding. Our data suggest that the plastic characteristics of α-catenin, revealed in response to both mechanical and biochemical cues, enable the functional-structural dynamics at the cellular and tissue levels. |
|
言語 |
en |
| 書誌情報 |
en : Scientific Reports
巻 6,
p. 24878,
発行日 2016-04-25
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
20452322 |
| 出版者 |
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出版者 |
Springer Nature |
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言語 |
en |
| 備考 |
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言語 |
ja |
|
値 |
Supplementary Information : srep_6_24878_s1.pdf |
| 権利情報 |
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言語 |
en |
|
権利情報 |
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| EID |
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識別子 |
332100 |
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識別子タイプ |
URI |
| 言語 |
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言語 |
eng |
srep_6_24878.pdf (2.1 MB)
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