| Item type |
文献 / Documents(1) |
| 公開日 |
2018-11-12 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1038/srep39967 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1038/srep39967 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
SHG-specificity of cellular Rootletin filaments enables naïve imaging with universal conservation |
|
言語 |
en |
| 著者 |
アキヤマ, トシヒロ
イノコ, アキヒト
カジ, ユウイチ
米村, 重信
カキグチ, キサ
セガワ, ヒロキ
イシツカ, ケイ
ヨシダ, マサキ
ヌマタ, オサム
Leproux, Philippe
Couderc, Vincent
オオシカ, テツロウ
カノウ, ヒデアキ
|
| 抄録 |
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|
内容記述タイプ |
Abstract |
|
内容記述 |
Despite growing demand for truly naïve imaging, label-free observation of cilium-related structure remains challenging, and validation of the pertinent molecules is correspondingly difficult. In this study, in retinas and cultured cells, we distinctively visualized Rootletin filaments in rootlets in the second harmonic generation (SHG) channel, integrated in custom coherent nonlinear optical microscopy (CNOM) with a simple, compact, and ultra-broadband supercontinuum light source. This SHG signal was primarily detected on rootlets of connecting cilia in the retinal photoreceptor and was validated by colocalization with anti-Rootletin staining. Transfection of cells with Rootletin fragments revealed that the SHG signal can be ascribed to filaments assembled from the R234 domain, but not to cross-striations assembled from the R123 domain. Consistent with this, Rootletin-depleted cells lacked SHG signal expected as centrosome linker. As a proof of concept, we confirmed that similar fibrous SHG was observed even in unicellular ciliates. These findings have potential for broad applications in clinical diagnosis and biophysical experiments with various organisms. |
|
言語 |
en |
| 書誌情報 |
en : Scientific Reports
巻 7,
p. 39967,
発行日 2017-01-06
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20452322 |
| 出版者 |
|
|
出版者 |
Springer Nature |
|
言語 |
en |
| 備考 |
|
|
言語 |
ja |
|
値 |
Supplementary Information : srep_7_39967_s1.pdf |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
© The Author(s) 2017 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| EID |
|
|
識別子 |
332131 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
srep_7_39967.pdf (2.37 MB)
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