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Development of versatile non-homologous end joining-based knock-in module for genome editing
https://tokushima-u.repo.nii.ac.jp/records/2005550
https://tokushima-u.repo.nii.ac.jp/records/200555049967540-6199-4707-a95b-5879f37bee1f
| 名前 / ファイル | ライセンス | アクション |
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srep_8_593.pdf (4.28 MB)
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srep_8_593_s1.pdf (1.81 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||||||||
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| 公開日 | 2018-11-12 | |||||||||||||||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1038/s41598-017-18911-9 | |||||||||||||||||||||||||||||
| 関連名称 | 10.1038/s41598-017-18911-9 | |||||||||||||||||||||||||||||
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| 出版タイプ | VoR | |||||||||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||||||||
| タイトル | Development of versatile non-homologous end joining-based knock-in module for genome editing | |||||||||||||||||||||||||||||
| 著者 |
沢津橋, 俊
× 沢津橋, 俊
WEKO
717
× ジョウコウ, ユウダイ
× 福本, 誠二× 松本, 俊夫
WEKO
1591
× 菅野, 茂夫
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| 内容記述 | CRISPR/Cas9-based genome editing has dramatically accelerated genome engineering. An important aspect of genome engineering is efficient knock-in technology. For improved knock-in efficiency, the non-homologous end joining (NHEJ) repair pathway has been used over the homology-dependent repair pathway, but there remains a need to reduce the complexity of the preparation of donor vectors. We developed the versatile NHEJ-based knock-in module for genome editing (VIKING). Using the consensus sequence of the time-honored pUC vector to cut donor vectors, any vector with a pUC backbone could be used as the donor vector without customization. Conditions required to minimize random integration rates of the donor vector were also investigated. We attempted to isolate null lines of the VDR gene in human HaCaT keratinocytes using knock-in/knock-out with a selection marker cassette, and found 75% of clones isolated were successfully knocked-in. Although HaCaT cells have hypotetraploid genome composition, the results suggest multiple clones have VDR null phenotypes. VIKING modules enabled highly efficient knock-in of any vectors harboring pUC vectors. Users now can insert various existing vectors into an arbitrary locus in the genome. VIKING will contribute to low-cost genome engineering. | |||||||||||||||||||||||||||||
| 書誌情報 |
en : Scientific Reports 巻 8, p. 593, 発行日 2018-01-12 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||||||||
| 収録物識別子 | 20452322 | |||||||||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||||||||
| 出版者 | Springer Nature | |||||||||||||||||||||||||||||
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| 値 | Supplementary information : srep_8_593_s1.pdf | |||||||||||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||||||||||
| 権利情報 | © The Author(s) 2018 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | |||||||||||||||||||||||||||||
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| 識別子 | 348363 | |||||||||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||||||||
