| Item type |
文献 / Documents(1) |
| 公開日 |
2018-11-12 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1038/ncomms12756 |
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言語 |
ja |
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関連名称 |
10.1038/ncomms12756 |
| 出版タイプ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Protein kinase D regulates positive selection of CD4+ thymocytes through phosphorylation of SHP-1 |
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言語 |
en |
| 著者 |
イシカワ, エリ
小迫, 英尊
ヤスダ, トモハル
オオムラヤ, マサキ
アラキ, キミ
クロサキ, トモヒロ
サイトウ, タカシ
ヤマサキ, ショウ
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Thymic selection shapes an appropriate T cell antigen receptor (TCR) repertoire during T cell development. Here, we show that a serine/threonine kinase, protein kinase D (PKD), is crucial for thymocyte positive selection. In T cell-specific PKD-deficient (PKD2/PKD3 double-deficient) mice, the generation of CD4 single positive thymocytes is abrogated. This defect is likely caused by attenuated TCR signalling during positive selection and incomplete CD4 lineage specification in PKD-deficient thymocytes; however, TCR-proximal tyrosine phosphorylation is not affected. PKD is activated in CD4+CD8+ double positive (DP) thymocytes on stimulation with positively selecting peptides. By phosphoproteomic analysis, we identify SH2-containing protein tyrosine phosphatase-1 (SHP-1) as a direct substrate of PKD. Substitution of wild-type SHP-1 by phosphorylation-defective mutant (SHP-1S557A) impairs generation of CD4+ thymocytes. These results suggest that the PKD–SHP-1 axis positively regulates TCR signalling to promote CD4+ T cell development. |
|
言語 |
en |
| 書誌情報 |
en : Nature Communications
巻 7,
p. 12756,
発行日 2016-09-27
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
20411723 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12645905 |
| 出版者 |
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出版者 |
Springer Nature |
|
言語 |
en |
| 備考 |
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言語 |
ja |
|
値 |
Supplementary Figures : ncomms_7_12756_s1.pdf
Supplementary Data : ncomms_7_12756_s2.xls, ncomms_7_12756_s3.xls, ncomms_7_12756_s4.xls |
| 権利情報 |
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|
言語 |
en |
|
権利情報 |
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| EID |
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|
識別子 |
320794 |
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識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
ncomms_7_12756.pdf (1.83 MB)
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ncomms_7_12756_s2.xls (63 KB)
