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Bactericidal action of ferulic acid with ultraviolet-A light irradiation
https://tokushima-u.repo.nii.ac.jp/records/2005973
https://tokushima-u.repo.nii.ac.jp/records/20059730b6f8e9e-cbe5-411e-a284-f32728549e29
| 名前 / ファイル | ライセンス | アクション |
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jphotobiol_191_52.pdf (2.08 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||
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| 公開日 | 2018-12-21 | |||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||
| 出版社版DOI | ||||||||||||||||||
| 関連識別子 | https://doi.org/10.1016/j.jphotobiol.2018.12.003 | |||||||||||||||||
| 関連名称 | 10.1016/j.jphotobiol.2018.12.003 | |||||||||||||||||
| 出版タイプ | ||||||||||||||||||
| 出版タイプ | AM | |||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Bactericidal action of ferulic acid with ultraviolet-A light irradiation | |||||||||||||||||
| 著者 |
白井, 昭博
× 白井, 昭博
WEKO
1235
× ヤストモ, ユウコ
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| 抄録 | ||||||||||||||||||
| 内容記述 | The bactericidal activity of ferulic acid (FA) against various microorganisms was remarkably enhanced by ultraviolet-A (UV-A) irradiation (wavelength, 365 nm). However, the bactericidal mechanism in the photo-combination system has not been evaluated. In the present study, this combined treatment was characterized by investigating associated changes in cellular functions of Escherichia coli, including assessments of respiratory activity, lipid peroxidation, membrane permeability, and damage to DNA and the cell surface. FA adsorbed onto and was incorporated into bacterial membranes, and the affinity resulted in decreased respiratory activity and enhanced lipid peroxidation in the cytoplasmic membrane with low- fluence (1.0 J/cm2) UV-A irradiation. Flow cytometry analysis revealed that additional exposure (8 J/cm2) combined with FA (1 mg/mL) induced increased cell permeability, yielding a 4.8- log decrease in the viable cell count. Morphologically, the treated cells exhibited a bacterial membrane dysfunction, producing many vesicles on the cell surface. However, despite this effect on the cell surface, plasmid DNA transformed into FA-treated E. coli maintained supercoiled integrity with negligible DNA oxidation. Our data strongly suggested that FA functions inside and outside the bacterial membrane; UV-A exposure in the presence of FA then causes increased oxidative modification and subsequent disruption of the bacterial membrane, without causing detectable genotoxicity. | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題 | Bactericidal action | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題 | Ferulic acid | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題 | Ultraviolet-A | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題 | Oxidative stress | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題 | Membrane damage | |||||||||||||||||
| 書誌情報 |
en : Journal of Photochemistry and Photobiology B: Biology 巻 191, p. 52-58, 発行日 2018-12-10 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 10111344 | |||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||
| 収録物識別子 | AA10686173 | |||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||
| 収録物識別子 | AA11533983 | |||||||||||||||||
| 出版者 | ||||||||||||||||||
| 出版者 | Elsevier | |||||||||||||||||
| 権利情報 | ||||||||||||||||||
| 権利情報 | © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||||||||||||||
| EID | ||||||||||||||||||
| 識別子 | 348568 | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | eng | |||||||||||||||||
